Latest revision as of 19:04, 26 July 2008

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Things we did today

Margaret

Grace

EtBr stained 1.2% agarose gel. Twenty microliters of each restriction digest reaction were loaded into each well.

Plasmid prep for nir+B0034, GFP+B0024, GFPf+B0024
Digested today's plasmid preps and E0026 and J33207 with EcoRI. Ran on an EtBr stained 1.2% gel to verify. (E0026 and J33207 may have been mixed up as we realized when sequencing returned CFP as the result for the lac part.)

The newly labeled E0026 (plasmid+part=2841bp) and J33207 (2679bp) plasmid preps are okay but what are the four bands? Linear, ?, ?, supercoiled.
GFP+B0034 (2874bp) and GFPf+B0034 (2872bp) are too small on the gel. GFP(f) didn't go in? Bands look ~2100bp.

Grace

All sequence reads are good (forward and/or reverse is of good enough quality to be a significant match against the original sequence) except for BB-pRL1383a. Need to reconstruct BB-pRL1383a because SpeI is the only BioBrick site identified and the insert is not that of BBa_B0034 (Could not ID what it was; blast search returned no results).

Margaret

I annotated some pictures and posted some finished experiments plus the plans for Construction of pRL1383a.

Margaret

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 7: / Line 7: @@
 :* The results are [[Team:Hawaii/Notebook/2008-07-23|here]]
+===Plasmid prep===
+:<strong> Grace</strong>
+[[Image:plasmidpreps072508.jpeg|right|thumb|200px|EtBr stained 1.2% agarose gel. Twenty microliters of each restriction digest reaction were loaded into each well.]]
+:* Plasmid prep for nir+B0034, GFP+B0024, GFPf+B0024
+:* Digested today's plasmid preps and E0026 and J33207 with EcoRI. Ran on an EtBr stained 1.2% gel to verify. (E0026 and J33207 may have been mixed up as we realized when sequencing returned CFP as the result for the lac part.)
+::* The newly labeled E0026 (plasmid+part=2841bp) and J33207 (2679bp) plasmid preps are okay but what are the four bands? Linear, ?, ?, supercoiled.
+::* GFP+B0034 (2874bp) and GFPf+B0034 (2872bp) are too small on the gel. GFP(f) didn't go in? Bands look ~2100bp.
 == Drylab Work ==