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Team:Hawaii/Notebook/2008-07-25
From 2008.igem.org
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:* Plasmid prep for nir+B0034, GFP+B0024, GFPf+B0024 | :* Plasmid prep for nir+B0034, GFP+B0024, GFPf+B0024 | ||
:* Digested today's plasmid preps and E0026 and J33207 with EcoRI. Ran on an EtBr stained 1.2% gel to verify. (E0026 and J33207 may have been mixed up as we realized when sequencing returned CFP as the result for the lac part.) | :* Digested today's plasmid preps and E0026 and J33207 with EcoRI. Ran on an EtBr stained 1.2% gel to verify. (E0026 and J33207 may have been mixed up as we realized when sequencing returned CFP as the result for the lac part.) | ||
| + | ::* The newly labeled E0026 (plasmid+part=2841bp) and J33207 (2679bp) plasmid preps are okay but what are the four bands? Linear, ?, ?, supercoiled. | ||
| + | ::* GFP+B0034 (2874bp) and GFPf+B0034 (2872bp) are too small on the gel. GFP(f) didn't go in? Bands look ~2100bp. | ||
== Drylab Work == | == Drylab Work == | ||
Latest revision as of 19:04, 26 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Checked Re-streaked Transformation
- Margaret
- The results are here
Plasmid prep
- Grace
- Plasmid prep for nir+B0034, GFP+B0024, GFPf+B0024
- Digested today's plasmid preps and E0026 and J33207 with EcoRI. Ran on an EtBr stained 1.2% gel to verify. (E0026 and J33207 may have been mixed up as we realized when sequencing returned CFP as the result for the lac part.)
- The newly labeled E0026 (plasmid+part=2841bp) and J33207 (2679bp) plasmid preps are okay but what are the four bands? Linear, ?, ?, supercoiled.
- GFP+B0034 (2874bp) and GFPf+B0034 (2872bp) are too small on the gel. GFP(f) didn't go in? Bands look ~2100bp.
Drylab Work
Sequencing Analysis
- Grace
- All sequence reads are good (forward and/or reverse is of good enough quality to be a significant match against the original sequence) except for BB-pRL1383a. Need to reconstruct BB-pRL1383a because SpeI is the only BioBrick site identified and the insert is not that of BBa_B0034 (Could not ID what it was; blast search returned no results).
Updating Wiki Experiments
- Margaret
- I annotated some pictures and posted some finished experiments plus the plans for Construction of pRL1383a.
Plans for making the pRL1383a construct an inducible high copy number
Margaret
- Started by annotating pSB2K3 on Vector NTI
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
