Team:UNIPV-Pavia/Notebook/Week10
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*We also infected 9 ml LB + Amp with 30 µl of BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' and '''BBa_B0030'''-BBa_C0061 glycerol stocks to grow two overnight cultures. | *We also infected 9 ml LB + Amp with 30 µl of BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' and '''BBa_B0030'''-BBa_C0061 glycerol stocks to grow two overnight cultures. | ||
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Revision as of 13:08, 27 July 2008
Home | The Team | The Project | Biological Safety | Parts Submitted to the Registry |
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Notebook
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
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Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 10: 07/21/08 - 07/25/08
07/21/08
- Colony PCR for BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079: 6 colonies from single colonies plate.
- Gel results: all the 6 picked colonies were true positive! we chose the 5th colont to grow a 9 ml overnight culture.
- We also infected 9 ml LB + Amp with 30 µl of BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 and BBa_B0030-BBa_C0061 glycerol stocks to grow two overnight cultures.
- Ligation: