"
Team:UNIPV-Pavia/Notebook/Week10
From 2008.igem.org
(Difference between revisions)
| Line 59: | Line 59: | ||
**BBa_J23100-BBa_B0030-BBa_C0012-'''BBa_B1006''' | **BBa_J23100-BBa_B0030-BBa_C0012-'''BBa_B1006''' | ||
**BBa_B0030-BBa_I15009-BBa_BBa_B1006-'''BBa_R0082''' | **BBa_B0030-BBa_I15009-BBa_BBa_B1006-'''BBa_R0082''' | ||
| - | ** | + | **BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006'''-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062 |
| - | ** | + | **BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006'''-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006 |
| + | |||
| + | *We incubated ligations at 16°C overnight. | ||
Revision as of 13:14, 27 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 10: 07/21/08 - 07/25/08
07/21/08
- Colony PCR for BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079: 6 colonies from single colonies plate.
 |
- Gel results: all the 6 picked colonies were true positive! we chose the 5th colont to grow a 9 ml overnight culture.
- We also infected 9 ml LB + Amp with 30 µl of BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 and BBa_B0030-BBa_C0061 glycerol stocks to grow two overnight cultures.
- Ligation:
- BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006
- BBa_B0030-BBa_I15009-BBa_BBa_B1006-BBa_R0082
- BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062
- BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
- We incubated ligations at 16°C overnight.
