Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(→Parts stored in the fridge in the lab (4°C))
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|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 30||213.6||14||July 29
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 30||213.6||14||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 48||311.0||31||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 40||214.0||47||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 40||209.5||48||July 29
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||15||July 23
|[http://partsregistry.org/Part:BBa_R0084 R0084]||pSB1A2||OmpF promoter||+/- 40||32.0||15||July 23
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|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||146.4||49||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||203.1||50||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||3d lock||+/- 35||117.4||23||July 29
|[http://partsregistry.org/Part:BBa_J23032 J23032]||BBa_J23006 (Ap)||3d lock||+/- 35||117.4||23||July 29
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|[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||3d key+ terminator||+/- 46||308.3||29||July 29
|[http://partsregistry.org/Part:BBa_J23022 J23022]||BBa_J23006 (Ap)||3d key+ terminator||+/- 46||308.3||29||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_B0015 B0015]||BBa_J52017 (Ap,Km)||Terminator||+/- 48||311.0||31||July 29
+
|[http://partsregistry.org/Part:BBa_J23109 J23109]||BBa_J61002 (Ap)||Constitutive Promoter||+/- 46||375.1||26||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_J23109 J23109]||BBa_J61002 (Ap)||Constitutive Promotor||+/- 46||375.1||26||July 29
+
|[http://partsregistry.org/Part:BBa_J23109 J23109]||BBa_J61002 (Ap)||Constitutive Promoter||+/- 48||404||30||July 29
-
|- style="background: #E0FFFF; text-align: center;" 
+
-
|[http://partsregistry.org/Part:BBa_J23109 J23109]||BBa_J61002 (Ap)||constitutive Pr||+/- 48||404||30||July 29
+
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_I712074 I712074]||BBa_J52017 (Ap,Km)||T7 Promotor||+/- 42.5||208.2||27||July 29
|[http://partsregistry.org/Part:BBa_I712074 I712074]||BBa_J52017 (Ap,Km)||T7 Promotor||+/- 42.5||208.2||27||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 30||104.0||22||July 29
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 30||104.0||22||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||170.8||45||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||159.0||46||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0062 R0062]||pSB1A2||LuxR regulated promoter ||+/- 40||104.1||37||July 31
|[http://partsregistry.org/Part:BBa_R0062 R0062]||pSB1A2||LuxR regulated promoter ||+/- 40||104.1||37||July 31
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
 +
|[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter||+/- 45||64.0||43||July 29
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter ||+/- 40||69.8||44||July 29
 +
|- style="background: #E0FFFF; text-align: center;"
|[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Cell Death||+/- 48||119.1||38||August 06
|[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Ligation for Cell Death||+/- 48||119.1||38||August 06
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
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|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Cell Death||+/- 46||41.0||42||August 06
|[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]||pSB1A2||Ligation for Cell Death||+/- 46||41.0||42||August 06
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter||+/- 45||64.0||43||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_R0011 R0011]||pSB1A2||LacI regulated promoter ||+/- 40||69.8||44||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||170.8||45||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||159.0||46||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 40||214.0||47||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_B0015 B0015]||pSB1AK3||Terminator||+/- 40||209.5||48||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||146.4||49||July 29
 
-
|- style="background: #E0FFFF; text-align: center;" 
 
-
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||203.1||50||July 29
 
|}
|}
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|}
|}
-
== Glycerol stock of cells (-80°C) ==
+
== Things stored in freezer in lab (-20°C) ==
 +
 
 +
 
 +
{|
 +
|- style="background: #003E81;color:white;width=100%"
 +
! colspan="6" style="background: #003E81" | Miscellaneous
 +
|- style="background: #99CCFF; text-align: center; width=100%"
 +
|width=160| '''Description'''
 +
|width=160| '''Name'''
 +
|width=160| '''µl left'''
 +
|width=160| '''Concentration'''
 +
|width=160| '''Box'''
 +
|width=160| '''Date'''
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer||1084||990||20 µM||1||August 01
 +
|- style="background: #E0FFFF; text-align: center;" 
 +
|Primer||1085||990||20 µM||2||August 01
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|dNTP mix||ATP, GTP, TTP, CTP||990||10 mM||3||August 01
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer||1084||500||100 µM||4||August 01
 +
|- style="background: #E0FFFF; text-align: center;"
 +
|Primer||1085||500||100 µM||5||August 01
 +
|}

Revision as of 12:08, 6 August 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 45303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30213.614July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40214.047July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.548July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 10114.818July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.449July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.150July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 30104.022July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 40170.845July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 40159.046July 29
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.137July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.043July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.844July 29
[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 48119.138August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48131.039August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48133.240August 06
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Reset+/- 48118.541August 06
[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Cell Death+/- 4641.042August 06


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677257.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125286.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62233.47July 29
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.39July 31
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector2077204.010July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33033111.711July 31
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.512July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.513July 30
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.714August 01
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert806158.215August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid20791718.216August 01
[http://partsregistry.org/Part:BBa_P1010 P1010] EcoR ISpe IInsert682104.917August 01
GFP-LVA EcoR ISpe IInsert625136.318August 05
[http://partsregistry.org/Part:BBa_C0012 C0012] EcoR ISpe IInsert1145255.619August 05

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer108499020 µM1August 01
Primer108599020 µM2August 01
dNTP mixATP, GTP, TTP, CTP99010 mM3August 01
Primer1084500100 µM4August 01
Primer1085500100 µM5August 01
Retrieved from "http://2008.igem.org/Team:KULeuven/Freezer"