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Team:Waterloo/Notebook
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==Notebook== | ==Notebook== | ||
| - | This year we are using a [http://www.google.com/notebook/public/05889908560840673300/BDRepIgoQp4H14akj Google Notebook] to co-ordinate our lab activities among team members. | + | This year we are using a [http://www.google.com/notebook/public/05889908560840673300/BDRepIgoQp4H14akj Google Notebook] to co-ordinate our lab activities among team members. This includes our day to day activities and our progress. |
| + | |||
| + | <b><u>Tentative Plans</u></b> | ||
| + | |||
| + | <b>Notes:</b><br> | ||
| + | <pre>' </pre> denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene <br> | ||
| + | <pre>'' </pre>denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)<br> | ||
| + | |||
| + | <b>Construction Stages </b><br> | ||
| + | 1) Construction of CI'' <br> | ||
| + | 2) Construction of Plac CI''<br> | ||
| + | 3) Ligate Plac (reverse) TT and Plac CI''<br> | ||
| + | |||
| + | <b>CI Repression Test</b><br> | ||
| + | 1) Construct Pconst CI''<br> | ||
| + | 2) Construct PR CI rfp''<br> | ||
| + | 3) Ligate above two constructs together (Pconst CI'' PR CI rfp'')<br> | ||
| + | 4) Test above repression by CI<br> | ||
| + | |||
| + | <b>Synthesize</b><br> | ||
| + | 1) Plac (reverse) TT<br> | ||
| + | 2) TT (reverse) PR<br> | ||
Revision as of 16:54, 15 August 2008
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Notebook
This year we are using a [http://www.google.com/notebook/public/05889908560840673300/BDRepIgoQp4H14akj Google Notebook] to co-ordinate our lab activities among team members. This includes our day to day activities and our progress.
Tentative Plans
Notes:
'denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene
''denotes that the construct contians the ribosome binding site, coding region for that gene, and a transcriptional terminator (TT)
Construction Stages
1) Construction of CI
2) Construction of Plac CI
3) Ligate Plac (reverse) TT and Plac CI
CI Repression Test
1) Construct Pconst CI
2) Construct PR CI rfp
3) Ligate above two constructs together (Pconst CI PR CI rfp)
4) Test above repression by CI
Synthesize
1) Plac (reverse) TT
2) TT (reverse) PR
