"
Edinburgh/9 July 2008
From 2008.igem.org
(Difference between revisions)
(→Wednesday 9 July 08) |
|||
| Line 333: | Line 333: | ||
* Results of L6 (BABEL2+''glgC''-mut1) and L7 (BABEL2+''glgC''-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to '''plates 26 and 27'''. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be ''glgC'' with EcoRI site 2 mutated out. | * Results of L6 (BABEL2+''glgC''-mut1) and L7 (BABEL2+''glgC''-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to '''plates 26 and 27'''. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be ''glgC'' with EcoRI site 2 mutated out. | ||
| - | * Minipreps '''M13 to M18''' of possible pSB1A2+''appY' transformants. Digests with EcoRI/PstI were run on '''Gel 10'''. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good). | + | * Minipreps '''M13 to M18''' of possible pSB1A2+''appY'' transformants. Digests with EcoRI/PstI were run on '''Gel 10'''. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good). |
* Digests and ligation '''L8''' for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as '''BBa_K118000''').<br /> | * Digests and ligation '''L8''' for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as '''BBa_K118000''').<br /> | ||
<br /> | <br /> | ||
:::: '''[[Edinburgh/10_July_2008|Next Entry >]]''' | :::: '''[[Edinburgh/10_July_2008|Next Entry >]]''' | ||
Revision as of 11:56, 21 August 2008
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Week 4
Wednesday 9 July 08
- Results of L6 (BABEL2+glgC-mut1) and L7 (BABEL2+glgC-mut2) transformations: no colonies for L6, but 9 colonies were obtained from L7. These were subcultured to plates 26 and 27. By the end of the day, these had grown sufficiently for 6 overnight cultures to be set up for minipreps tomorrow. In theory, some or all of these should be glgC with EcoRI site 2 mutated out.
- Minipreps M13 to M18 of possible pSB1A2+appY transformants. Digests with EcoRI/PstI were run on Gel 10. All but M13 looked OK (insert band on M14 was so faint as to be ambiguous, but M15 to M18 all look good).
- Digests and ligation L8 for addition of a synthetic ribosome binding site M2 (which has now been entered into the Registry as BBa_K118000).
