"
Team:Paris/Modeling/f4
From 2008.igem.org
(Difference between revisions)
| Line 74: | Line 74: | ||
|- | |- | ||
|n<sub>3</sub> | |n<sub>3</sub> | ||
| - | |complexation order of | + | |complexation order of FlhDC-pFliA <br> n<sub>3</sub> |
|no dimension | |no dimension | ||
| | | | ||
| Line 85: | Line 85: | ||
|- | |- | ||
|(K<sub>9</sub>/{coef<sub>fliA</sub>}<sup>n<sub>9</sub></sup>) | |(K<sub>9</sub>/{coef<sub>fliA</sub>}<sup>n<sub>9</sub></sup>) | ||
| - | |activation constant of | + | |activation constant of FliA-pFliA <br> K<sub>9</sub> |
|nM<sup>n<sub>9</sub></sup> | |nM<sup>n<sub>9</sub></sup> | ||
| | | | ||
|- | |- | ||
|n<sub>9</sub> | |n<sub>9</sub> | ||
| - | |complexation order of | + | |complexation order of FliA-pFliA <br> n<sub>19</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 16:29, 21 August 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFliA)] | expression rate of pFliA with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β5 | production rate of FlhDC-pFliA with RBS E0032 β5 | nM.s-1 | |
| (K3/{coefflhDC}n3) | activation constant of FlhDC-pFliA K3 | nMn3 | |
| n3 | complexation order of FlhDC-pFliA n3 | no dimension | |
| β13 | production rate of FliA-pFliA with RBS E0032 β13 | nM.s-1 | |
| (K9/{coeffliA}n9) | activation constant of FliA-pFliA K9 | nMn9 | |
| n9 | complexation order of FliA-pFliA n19 | no dimension |
Then, if we have time, we want to verify the expected relation
