|
Method & Algorithm : ƒ4
= act_pFliA
 Specific Plasmid Characterisation for ƒ4 According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state,
we have [FlhDC]real = {coefflhDC} ƒ1([aTc]i)
and [FliA]real = {coeffliA} ƒ2([arab]i)
but we use [aTc]i = Inv_ƒ1( [FlhDC] )
and [arab]i = Inv_ƒ2( [FliA] )
So, at steady-states,
we use this analytical expression to determine the parameters :
↓ Table of Values ↑
| param
| signification
| unit
| value
| comments
|
| (fluorescence)
| value of the observed fluorescence
| au
|
| need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
|
| conversion
| conversion ratio between
fluorescence and concentration
↓ gives ↓
| nM.au-1
| (1/79.429)
|
|
| [GFP]
| GFP concentration at steady-state
| nM
|
|
|
| γGFP
| dilution-degradation rate
of GFP(mut3b)
↓ gives ↓
| min-1
| 0.0198
| Time Cell Division : 35 min.
|
| ƒ4
| activity of
pFliA with RBS E0032
| nM.min-1
|
|
|
| param
| signification
corresponding parameters in the equations
| unit
| value
| comments
|
| β18
| total transcription rate of
FlhDC><pFliA with RBS E0032
β18
| nM.min-1
|
|
|
| (K1/{coefflhDC})
| activation constant of FlhDC><pFliA
K1
| nM
|
|
|
| n1
| complexation order of FlhDC><pFliA
n1
| no dimension
|
|
|
| β23
| total transcription rate of
FliA><pFliA with RBS E0032
β23
| nM.min-1
|
|
|
| (K7/{coeffliA})
| activation constant of FliA><pFliA
K7
| nM
|
|
|
| n10
| complexation order of FliA><pFliA
n7
| no dimension
|
|
|
|
Then, if we have time, we want to verify the expected relation
<Back - to "Implementation" |
<Back - to "Protocol Of Characterization" |
|
"
