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Edinburgh/4 August 2008
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| - | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
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</ul> | </ul> | ||
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| - | === | + | <table> |
| - | ==== Monday 4 August 08 | + | <html> |
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| + | table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; } | ||
| + | table.month .dow td { color:#000000; text-align:center; font-size:100%; } | ||
| + | table.month td.today { background-color:#cd0000; } | ||
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| + | } | ||
| + | #bodyContent table.month a { background:none; padding:0 } | ||
| + | .day-active { color:#cd0000 } | ||
| + | .day-empty { color:#000000 } | ||
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| + | </style> | ||
| + | </html> | ||
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| + | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" | ||
| + | </table> | ||
| + | |||
| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | :::: '''[[Edinburgh/3_August_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 8 == | ||
| + | === Monday 4 August 08 === | ||
* Made 17 minipreps from transformations of L26, L27, L28, L29: '''M80-83''' (L26: rbs+''appY'' in Edinbrick1), '''M84-85''' (L27: rbs+''crtB'' in Edinbrick1), '''M86-90''' (L28: ''cenA'' in Edinbrick1) and '''M91-96''' (L29: ''cex'' in Edinbrick1). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG) | * Made 17 minipreps from transformations of L26, L27, L28, L29: '''M80-83''' (L26: rbs+''appY'' in Edinbrick1), '''M84-85''' (L27: rbs+''crtB'' in Edinbrick1), '''M86-90''' (L28: ''cenA'' in Edinbrick1) and '''M91-96''' (L29: ''cex'' in Edinbrick1). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG) | ||
* Third ''glgC'' mutagenesis made by MABEL on BABEL2+''glgC''-mut1,2 (since pSB1A2+''glgC''-mut1,2 appears to have failed) performed: '''P50''' (Yan) | * Third ''glgC'' mutagenesis made by MABEL on BABEL2+''glgC''-mut1,2 (since pSB1A2+''glgC''-mut1,2 appears to have failed) performed: '''P50''' (Yan) | ||
* Double digestions of M80 to M96 and P50 were run on '''gel 36'''. Results were good for M80-82 (pSB1A2+rbs+''appY''), M84-85 (pSB1A2+rbs+''crtB''), M92 (pSB1A2+''cex'') and P50 (''glgC''-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+''cenA''). (Yan, OG) | * Double digestions of M80 to M96 and P50 were run on '''gel 36'''. Results were good for M80-82 (pSB1A2+rbs+''appY''), M84-85 (pSB1A2+rbs+''crtB''), M92 (pSB1A2+''cex'') and P50 (''glgC''-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+''cenA''). (Yan, OG) | ||
| - | * Sequencing results back today indicate that M50 = pSB1A2+rbs+''crtI'', M63 = pSB1A2+rbs+''crtE'' and M67 = pSB1A2+rbs+''crtB''. (AH) | + | * Sequencing results back today indicate that M50 = pSB1A2+rbs+''crtI'', M63 = pSB1A2+rbs+''crtE'' and M67 = pSB1A2+rbs+''crtB''. (AH)<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/5_August_2008|Next Entry >]]''' | ||
Revision as of 10:20, 28 August 2008
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Week 8
Monday 4 August 08
- Made 17 minipreps from transformations of L26, L27, L28, L29: M80-83 (L26: rbs+appY in Edinbrick1), M84-85 (L27: rbs+crtB in Edinbrick1), M86-90 (L28: cenA in Edinbrick1) and M91-96 (L29: cex in Edinbrick1). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
- Third glgC mutagenesis made by MABEL on BABEL2+glgC-mut1,2 (since pSB1A2+glgC-mut1,2 appears to have failed) performed: P50 (Yan)
- Double digestions of M80 to M96 and P50 were run on gel 36. Results were good for M80-82 (pSB1A2+rbs+appY), M84-85 (pSB1A2+rbs+crtB), M92 (pSB1A2+cex) and P50 (glgC-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+cenA). (Yan, OG)
- Sequencing results back today indicate that M50 = pSB1A2+rbs+crtI, M63 = pSB1A2+rbs+crtE and M67 = pSB1A2+rbs+crtB. (AH)
