Edinburgh/4 August 2008

From 2008.igem.org

(Difference between revisions)
(→Monday 4 August 08)
Line 332: Line 332:
=== Monday 4 August 08 ===
=== Monday 4 August 08 ===
-
* Made 17 minipreps from transformations of L26, L27, L28, L29: '''M80-83''' (L26: rbs+''appY'' in Edinbrick1), '''M84-85''' (L27: rbs+''crtB'' in Edinbrick1), '''M86-90''' (L28: ''cenA'' in Edinbrick1) and '''M91-96''' (L29: ''cex'' in Edinbrick1). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
+
* Made 17 minipreps from colonies on plates 83-86: '''M80-83''' from plate 83 (L26: pSB1A2+rbs+''appY''), '''M84-85''' from plate 84 (L27: pSB1A2+rbs+''crtB''), '''M86-90''' from plate 85 (L28: pSB1A2+''cenA'') and '''M91-96''' from plate 86 (L29: pSB1A2+''cex''). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
-
* Third ''glgC'' mutagenesis made by MABEL on BABEL2+''glgC''-mut1,2 (since pSB1A2+''glgC''-mut1,2 appears to have failed) performed: '''P50''' (Yan)
+
* Third ''glgC'' mutagenesis performed by MABEL on BABEL2+''glgC''-mut1,2 (since pSB1A2+''glgC''-mut1,2 appears to have failed): '''P50''' (Yan)
-
* Double digestions of M80 to M96 and P50 were run on '''gel 36'''. Results were good for M80-82 (pSB1A2+rbs+''appY''), M84-85 (pSB1A2+rbs+''crtB''), M92 (pSB1A2+''cex'') and P50 (''glgC''-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+''cenA''). (Yan, OG)
+
* P50 and double digestions of M80-M96 were run on '''gel 36'''. Results were good for M80-82 (pSB1A2+rbs+''appY''), M84-85 (pSB1A2+rbs+''crtB''), M92 (pSB1A2+''cex'') and P50 (''glgC''-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+''cenA''). (Yan, OG)
-
* Sequencing results back today indicate that M50 = pSB1A2+rbs+''crtI'', M63 = pSB1A2+rbs+''crtE'' and M67 = pSB1A2+rbs+''crtB''. (AH)<br />
+
* Sequencing results back today indicate that M50 is pSB1A2+rbs+''crtI'', M63 is pSB1A2+rbs+''crtE'' and M67 is pSB1A2+rbs+''crtB''. (AH)<br />
<br />
<br />
:::: '''[[Edinburgh/5_August_2008|Next Entry >]]'''
:::: '''[[Edinburgh/5_August_2008|Next Entry >]]'''

Revision as of 10:40, 28 August 2008

Edinburgh iGEM 2008

 

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Edinburgh/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Edinburgh/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Edinburgh/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Edinburgh/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Edinburgh/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Edinburgh/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Edinburgh/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Edinburgh/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Edinburgh/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Edinburgh/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Edinburgh/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Edinburgh/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Edinburgh/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/16_June_2008&action=edit 16] [http://2008.igem.org/Edinburgh/17_June_2008 17] [http://2008.igem.org/wiki/index.php?title=Edinburgh/18_June_2008&action=edit 18] [http://2008.igem.org/Edinburgh/19_June_2008 19] [http://2008.igem.org/Edinburgh/20_June_2008 20] [http://2008.igem.org/wiki/index.php?title=Edinburgh/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Edinburgh/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Edinburgh/23_June_2008&action=edit 23] [http://2008.igem.org/Edinburgh/24_June_2008 24] [http://2008.igem.org/Edinburgh/25_June_2008 25] [http://2008.igem.org/Edinburgh/26_June_2008 26] [http://2008.igem.org/Edinburgh/27_June_2008 27] [http://2008.igem.org/Edinburgh/28_June_2008 28] [http://2008.igem.org/Edinburgh/29_June_2008 29]
[http://2008.igem.org/Edinburgh/30_June_2008 30]
July
MTWTFSS
  [http://2008.igem.org/Edinburgh/1_July_2008 1] [http://2008.igem.org/Edinburgh/2_July_2008 2] [http://2008.igem.org/Edinburgh/3_July_2008 3] [http://2008.igem.org/Edinburgh/4_July_2008 4] [http://2008.igem.org/Edinburgh/5_July_2008 5] [http://2008.igem.org/Edinburgh/6_July_2008 6]
[http://2008.igem.org/Edinburgh/7_July_2008 7] [http://2008.igem.org/Edinburgh/8_July_2008 8] [http://2008.igem.org/Edinburgh/9_July_2008 9] [http://2008.igem.org/Edinburgh/10_July_2008 10] [http://2008.igem.org/Edinburgh/11_July_2008 11] [http://2008.igem.org/Edinburgh/12_July_2008 12] [http://2008.igem.org/Edinburgh/13_July_2008 13]
[http://2008.igem.org/Edinburgh/14_July_2008 14] [http://2008.igem.org/Edinburgh/15_July_2008 15] [http://2008.igem.org/Edinburgh/16_July_2008 16] [http://2008.igem.org/Edinburgh/17_July_2008 17] [http://2008.igem.org/Edinburgh/18_July_2008 18] [http://2008.igem.org/Edinburgh/19_July_2008 19] [http://2008.igem.org/Edinburgh/20_July_2008 20]
[http://2008.igem.org/Edinburgh/21_July_2008 21] [http://2008.igem.org/Edinburgh/22_July_2008 22] [http://2008.igem.org/Edinburgh/23_July_2008 23] [http://2008.igem.org/Edinburgh/24_July_2008 24] [http://2008.igem.org/Edinburgh/25_July_2008 25] [http://2008.igem.org/Edinburgh/26_July_2008 26] [http://2008.igem.org/Edinburgh/27_July_2008 27]
[http://2008.igem.org/Edinburgh/28_July_2008 28] [http://2008.igem.org/Edinburgh/29_July_2008 29] [http://2008.igem.org/Edinburgh/30_July_2008 30] [http://2008.igem.org/Edinburgh/31_July_2008 31]
August
MTWTFSS
        [http://2008.igem.org/Edinburgh/1_August_2008 1] [http://2008.igem.org/wiki/index.php?title=Edinburgh/2_August_2008&action=edit 2] [http://2008.igem.org/Edinburgh/3_August_2008 3]
[http://2008.igem.org/Edinburgh/4_August_2008 4] [http://2008.igem.org/Edinburgh/5_August_2008 5] [http://2008.igem.org/Edinburgh/6_August_2008 6] [http://2008.igem.org/Edinburgh/7_August_2008 7] [http://2008.igem.org/Edinburgh/8_August_2008 8] [http://2008.igem.org/Edinburgh/9_August_2008 9] [http://2008.igem.org/Edinburgh/10_August_2008 10]
[http://2008.igem.org/Edinburgh/11_August_2008 11] [http://2008.igem.org/Edinburgh/12_August_2008 12] [http://2008.igem.org/Edinburgh/13_August_2008 13] [http://2008.igem.org/Edinburgh/14_August_2008 14] [http://2008.igem.org/Edinburgh/15_August_2008 15] [http://2008.igem.org/Edinburgh/16_August_2008 16] [http://2008.igem.org/Edinburgh/17_August_2008 17]
[http://2008.igem.org/Edinburgh/18_August_2008 18] [http://2008.igem.org/Edinburgh/19_August_2008 19] [http://2008.igem.org/Edinburgh/20_August_2008 20] [http://2008.igem.org/Edinburgh/21_August_2008 21] [http://2008.igem.org/Edinburgh/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Edinburgh/23_August_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Edinburgh/24_August_2008&action=edit 24]
[http://2008.igem.org/Edinburgh/25_August_2008 25] [http://2008.igem.org/Edinburgh/26_August_2008 26] [http://2008.igem.org/Edinburgh/27_August_2008 27] [http://2008.igem.org/Edinburgh/28_August_2008 28] [http://2008.igem.org/Edinburgh/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Edinburgh/30_August_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Edinburgh/31_August_2008&action=edit 31]
< Previous Entry

Week 8

Monday 4 August 08

  • Made 17 minipreps from colonies on plates 83-86: M80-83 from plate 83 (L26: pSB1A2+rbs+appY), M84-85 from plate 84 (L27: pSB1A2+rbs+crtB), M86-90 from plate 85 (L28: pSB1A2+cenA) and M91-96 from plate 86 (L29: pSB1A2+cex). This was followed by double digestion of all the miniprep products (M80-96) with EcoRI/PstI. (Yan, OG)
  • Third glgC mutagenesis performed by MABEL on BABEL2+glgC-mut1,2 (since pSB1A2+glgC-mut1,2 appears to have failed): P50 (Yan)
  • P50 and double digestions of M80-M96 were run on gel 36. Results were good for M80-82 (pSB1A2+rbs+appY), M84-85 (pSB1A2+rbs+crtB), M92 (pSB1A2+cex) and P50 (glgC-mut1,2,3). Other results were poor, with bands in the wrong position for M86-M90 (pSB1A2+cenA). (Yan, OG)
  • Sequencing results back today indicate that M50 is pSB1A2+rbs+crtI, M63 is pSB1A2+rbs+crtE and M67 is pSB1A2+rbs+crtB. (AH)


Next Entry >
Retrieved from "http://2008.igem.org/Edinburgh/4_August_2008"