Alberta NINT/20 August 2008

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             too low.
             too low.
             [[Image:NINT_SD_p101.jpg|750 px]]
             [[Image:NINT_SD_p101.jpg|750 px]]
 +
 +
lab work (JD):
 +
              Miniprep of O/N of cotransformed cells
 +
              Day 2 of LacZ Assay
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Revision as of 02:53, 4 September 2008

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lab work (SD): 

            Digested TA2+(PCR) with BamHI+NcoI.  Incubated at 37 C for 1 hr.
            Ran TA2+(PCR) (10ul) and TA2+(PCR)/B+N (10ul) on 2% agarose gel 110 V 60 min
            Used nucleotide removal purification kit to purify 10ul of TA2+(PCR)/B+N.
                    4.4 ng/ul 1.96 A260/280 67 bp = 99.499 fmol/ul
            The gel did not have a band for the 67 bp TA2+(PCR)/B+N.  This may be because the concentration is 
            too low.
            NINT SD p101.jpg

lab work (JD): 

             Miniprep of O/N of cotransformed cells
             Day 2 of LacZ Assay

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