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Team:Hawaii/Notebook/2008-09- 2
From 2008.igem.org
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:* 1 sleeve of LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> | :* 1 sleeve of LB+sp<sub>2.5</sub>+sm<sub>2.5</sub> | ||
:* 2 sleeves of LB+amp<sub>100</sub> | :* 2 sleeves of LB+amp<sub>100</sub> | ||
| + | |||
| + | ===Construction of GFP + TT=== | ||
| + | :<strong>Krystle</strong> | ||
| + | |||
| + | :* Ligated GFP (cut with SP) with tt (synthesized) using T4 ligase | ||
| + | :: negative control: re'd GFP ligated to self | ||
= Discussion = | = Discussion = | ||
Latest revision as of 20:09, 4 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace & Krystle
- BB-pRL1383a
- GFPf + tt colonies #8, 17, and 21
Colony PCR
- Grace
File:090208colonyPCR.jpg
EtBr stained 2% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.
- Colony PCR of 3A ligation transformants
PCR
- Grace
- J33207 with HindIII/BamHI primers
RE digest
- Grace
- J33207 and BB-pRL1383a with HindIII and BamHI
Made media plates
- Grace
- 1 sleeve of LB+sp2.5+sm2.5
- 2 sleeves of LB+amp100
Construction of GFP + TT
- Krystle
- Ligated GFP (cut with SP) with tt (synthesized) using T4 ligase
- negative control: re'd GFP ligated to self
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
