"
Team:BCCS-Bristol/Calendar-Notebook/22 July 2008
From 2008.igem.org
Rodgerread (Talk | contribs) |
Tgorochowski (Talk | contribs) |
||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | <html><link rel="stylesheet" href="http:// | + | <html><link rel="stylesheet" href="http://homepage.mac.com/tgorochowski/iGEM/bccs-igem.css" type="text/css"></html> |
__NOTOC__ | __NOTOC__ | ||
<div class="bccsNavBar"> | <div class="bccsNavBar"> | ||
| Line 15: | Line 15: | ||
</div> | </div> | ||
| + | ==Bead experiment 1== | ||
| + | It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact. The chamber was left under the microscope overnight (results see [[Team:BCCS-Bristol/Calendar-Notebook/23 July 2008 | 23 July 2008]]). | ||
| - | + | ==BioBrick Transformation== | |
| - | Carried on with biobrick transformation | + | Carried on with biobrick transformation of the [http://partsregistry.org/Part:BBa_E0240 GFP generator]. The DNA was transformed into ''E. coli'' DH5α cells along with pUC19 as a control using chemical competent cells and heat shock. |
| - | + | ||
| + | ==Swimming agar assay== | ||
| + | |||
| + | The chemotaxis experiment was continued, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before ([[Team:BCCS-Bristol/Calendar-Notebook/21 July 2008 | 21 July 2008]]). | ||
Latest revision as of 09:56, 14 September 2008
Bead experiment 1
It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact. The chamber was left under the microscope overnight (results see 23 July 2008).
BioBrick Transformation
Carried on with biobrick transformation of the [http://partsregistry.org/Part:BBa_E0240 GFP generator]. The DNA was transformed into E. coli DH5α cells along with pUC19 as a control using chemical competent cells and heat shock.
Swimming agar assay
The chemotaxis experiment was continued, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before ( 21 July 2008).
