Team:KULeuven/Project/Reset

From 2008.igem.org

(Difference between revisions)

Latest revision as of 14:51, 3 October 2008

dock/undock dropdown

Reset

BioBricks

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 BBa_I712074]) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 BBa_J23078]). The aiiA gene ([http://partsregistry.org/Part:BBa_C0060 BBa_C0060]), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.

Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.

@@ Line 1: / Line 1: @@
+{{:Team:KULeuven/Tools/Styling}}
+{{:Team:KULeuven/Tools/Scripting}}
 {{:Team:KULeuven/Tools/Header}}
-<div style="float: right;">[[Image:logo_reset.jpg|120px]]</div>
+[[Image:logo_reset.jpg|120px|right]]
 ==Reset==
@@ Line 10: / Line 12: @@
 ===Components===
-The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 '''BBa_J23078''']). The ''aiiA'' gene ([http://partsregistry.org/Part:BBa_C0060 '''BBa_C0060''']), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. This inactivates the 3OC6HSL autoinducer molecule, efficiently removing it from the active system. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])
+The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 '''BBa_J23078''']). The ''aiiA'' gene ([http://partsregistry.org/Part:BBa_C0060 '''BBa_C0060''']), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])
 ===Action===
-The reset mechanism is made to destroy all HSL at the time, when significant input re-emerges. Originally, a pulse of lactonase was thought to do the thing, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the concentration should be high enough and the device should work.
+This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter Timer].
-The ''aiiA'' gene coding for lactonase is regulated by the output signal from the filter, so that background input signal does not result in timer reset.
+When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.
+Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.
 {{:Team:KULeuven/Tools/Components}}