"
Team:KULeuven/1 August 2008
From 2008.igem.org
m |
|||
| (5 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:KULeuven/Tools/Styling}} | ||
| + | {{:Team:KULeuven/Tools/Header}} | ||
| + | |||
{{:Team:KULeuven/Tools/New_Day/Date_Retriever}} | {{:Team:KULeuven/Tools/New_Day/Date_Retriever}} | ||
| Line 4: | Line 7: | ||
=== Wet Lab === | === Wet Lab === | ||
| - | + | The ligated plasmids that were transformed into TOP10 cells yesterday, didn't give any colonies. We think that maybe the transfomation is the problem. So we wanted to use electroporation to transform the cells, but we ran out of competent cells. New competent TOP10 cells are inoculated and incubated overnight in the 37°C incubator. | |
| - | + | ||
| + | The ethanolprecipitation we started yesterday was continued today. After that, the parts were cut with ''EcoR''I , put on gel and purified. [http://partsregistry.org/Part:BBa_P1010 P1010], [http://partsregistry.org/Part:BBa_C0060 C0060] and [http://partsregistry.org/Part:BBa_C0062 C0062] were properly cut, [http://partsregistry.org/Part:BBa_I712022 I712022] wasn't. The ligation of [http://partsregistry.org/Part:BBa_P1010 P1010], [http://partsregistry.org/Part:BBa_C0060 C0060] and [http://partsregistry.org/Part:BBa_C0062 C0062], all with [http://partsregistry.org/Part:BBa_B0015 B0015], was started and could continue overnight. | ||
| + | |||
| + | The primers to make GFP with LVA tag arrived today. So we did a PCR to amplify this part and to attach the prefix and suffix. GFP-LVA is now on its way to become a BioBrick (after ligation into a plasmid). | ||
=== Dry Lab === | === Dry Lab === | ||
| + | |||
| + | ==== General==== | ||
Primers, wiki... usual stuff. Made to-do list for Jonas. | Primers, wiki... usual stuff. Made to-do list for Jonas. | ||
| - | == Modeling == | + | ==== Modeling ==== |
| - | + | ||
Part one of the mathematical analysis of the memory is done: stability of the two stationary states is proven. For next week: try to find the boundary between the initial states going to one of the two stationary states. | Part one of the mathematical analysis of the memory is done: stability of the two stationary states is proven. For next week: try to find the boundary between the initial states going to one of the two stationary states. | ||
| - | |||
| - | |||
== Remarks == | == Remarks == | ||
| - | [[Image:pictogram_celldeath.png| | + | [[Image:pictogram_celldeath.png|300px|center|frame|Sweet kisses for Rachel]] |
Latest revision as of 13:11, 7 October 2008
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
The ligated plasmids that were transformed into TOP10 cells yesterday, didn't give any colonies. We think that maybe the transfomation is the problem. So we wanted to use electroporation to transform the cells, but we ran out of competent cells. New competent TOP10 cells are inoculated and incubated overnight in the 37°C incubator.
The ethanolprecipitation we started yesterday was continued today. After that, the parts were cut with EcoRI , put on gel and purified. [http://partsregistry.org/Part:BBa_P1010 P1010], [http://partsregistry.org/Part:BBa_C0060 C0060] and [http://partsregistry.org/Part:BBa_C0062 C0062] were properly cut, [http://partsregistry.org/Part:BBa_I712022 I712022] wasn't. The ligation of [http://partsregistry.org/Part:BBa_P1010 P1010], [http://partsregistry.org/Part:BBa_C0060 C0060] and [http://partsregistry.org/Part:BBa_C0062 C0062], all with [http://partsregistry.org/Part:BBa_B0015 B0015], was started and could continue overnight.
The primers to make GFP with LVA tag arrived today. So we did a PCR to amplify this part and to attach the prefix and suffix. GFP-LVA is now on its way to become a BioBrick (after ligation into a plasmid).
Dry Lab
General
Primers, wiki... usual stuff. Made to-do list for Jonas.
Modeling
Part one of the mathematical analysis of the memory is done: stability of the two stationary states is proven. For next week: try to find the boundary between the initial states going to one of the two stationary states.
Remarks
