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Team:KULeuven/14 August 2008
From 2008.igem.org
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=== Wet Lab === | === Wet Lab === | ||
| + | The PCR samples of the T7 polymerase with UmuD tag were put on gel. The results were not good. It is still a smear. | ||
| + | |||
| + | Some more ligation products were cut: [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] with ''Xba''I and ''EcoR''I; [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0062 R0062]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032] with ''Spe''I and ''EcoR''I. Only R0062+B0032 and J23100+B0032 were not properly cut. It is possible that the ligations were not correct. | ||
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| + | The ligations that failed were set up again: [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] , [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]. | ||
=== Dry Lab === | === Dry Lab === | ||
| - | == Modeling == | + | ==== Modeling ==== |
Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) | Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) | ||
| - | The wiki was also updated and remarks are all around, so a lot of work still to do | + | The wiki was also updated and remarks are all around, so a lot of work still to do. |
Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/ | Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/ | ||
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Anyhow, time for a long weekend. See you on monday! | Anyhow, time for a long weekend. See you on monday! | ||
| - | == Wiki == | + | ==== Wiki ==== |
Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added. | Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added. | ||
| - | |||
| - | |||
Latest revision as of 12:49, 11 October 2008
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
The PCR samples of the T7 polymerase with UmuD tag were put on gel. The results were not good. It is still a smear.
Some more ligation products were cut: [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032], [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] with XbaI and EcoRI; [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0062 R0062]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032] with SpeI and EcoRI. Only R0062+B0032 and J23100+B0032 were not properly cut. It is possible that the ligations were not correct.
The ligations that failed were set up again: [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] , [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015].
Dry Lab
Modeling
Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) The wiki was also updated and remarks are all around, so a lot of work still to do.
Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/
It's been said before this week, but things are looking quite good. We might have reached some sort of end point here where everything should be final. (hopefully)
Anyhow, time for a long weekend. See you on monday!
Wiki
Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added.
