Alberta NINT/3 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
- | [[Alberta_NINT/2_July_2008 | < Previous entry]] | + | [[Alberta_NINT/2_July_2008 | < Previous entry]] | [[Alberta_NINT/4_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08. | Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08. | ||
- | [[ | + | lab work (JD): |
+ | From sequencing K102006.4 becomes official K10200 and K102007.5 becomes official K102007 | ||
+ | Digested pSB4A5 with EcoR1 and Pst1 | ||
+ | Digested K102006 and K102003 with EcoR1 and Pst1 | ||
+ | Ran 2% agarose gel electrophoresis | ||
+ | Made glycerol stock of K102006 and K102007 | ||
+ | [[Image:annotated5.jpg]] |
Latest revision as of 22:56, 11 October 2008
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lab work (SD):
Sequenced pSB4A5 to verify. Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08.
lab work (JD):
From sequencing K102006.4 becomes official K10200 and K102007.5 becomes official K102007 Digested pSB4A5 with EcoR1 and Pst1 Digested K102006 and K102003 with EcoR1 and Pst1 Ran 2% agarose gel electrophoresis Made glycerol stock of K102006 and K102007