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Alberta NINT/15 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/14_July_2008 | < Previous entry]] | + | [[Alberta_NINT/14_July_2008 | < Previous entry]] | [[Alberta_NINT/16_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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K102032+K102036 (LB+amp50+kan35) | K102032+K102036 (LB+amp50+kan35) | ||
| - | + | lab work (JD): | |
| + | We've decided to re-do the ligation another time from scratch: | ||
| + | Created O/N from glycerol stocks of: | ||
| + | pSB4A5 | ||
| + | K102003 | ||
| + | K102006 | ||
Latest revision as of 22:59, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Since the double-plasmid cell cultures failed to grow in LB+amp50+kan35 broth, perhaps the colonies
grown on LB+amp50+kan35 plates were not selected for kan resistance (ie. kan was not present on
these plates). New LB+amp50+kan35 plates were made and the transformation was redone.
K102022 (LB+amp50)
K102032 (LB+amp50)
K102032+K102011 (LB+amp50+kan35)
K102032+K102034 (LB+amp50+kan35)
K102032+K102035 (LB+amp50+kan35)
K102032+K102036 (LB+amp50+kan35)
lab work (JD):
We've decided to re-do the ligation another time from scratch:
Created O/N from glycerol stocks of:
pSB4A5
K102003
K102006
