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Alberta NINT/20 August 2008
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The gel did not have a band for the 67 bp TA2+(PCR)/B+N. This may be because the concentration is | The gel did not have a band for the 67 bp TA2+(PCR)/B+N. This may be because the concentration is | ||
too low. | too low. | ||
| + | [[Image:NINT_SD_p101.jpg|750 px]] | ||
| - | + | lab work (JD): | |
| + | Miniprep of O/N of cotransformed cells | ||
| + | Day 2 of LacZ Assay | ||
Latest revision as of 23:10, 11 October 2008
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lab work (SD):
Digested TA2+(PCR) with BamHI+NcoI. Incubated at 37 C for 1 hr.
Ran TA2+(PCR) (10ul) and TA2+(PCR)/B+N (10ul) on 2% agarose gel 110 V 60 min
Used nucleotide removal purification kit to purify 10ul of TA2+(PCR)/B+N.
4.4 ng/ul 1.96 A260/280 67 bp = 99.499 fmol/ul
The gel did not have a band for the 67 bp TA2+(PCR)/B+N. This may be because the concentration is
too low.
lab work (JD):
Miniprep of O/N of cotransformed cells
Day 2 of LacZ Assay
