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Team:KULeuven/1 September 2008
From 2008.igem.org
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== Lab Work == | == Lab Work == | ||
=== Wet Lab === | === Wet Lab === | ||
| - | + | We did a lot of PCRs today. We tried to add the second part of the UmuD tag with the 91bp-primer. We had very sharp and extremely beautiful bands, but they seemed three times too long: FAIL. This will be done again tomorrow, with primers 2.1 and 2.2. The antisense LuxI PCR resulted in a smear: FAIL again. To be done again tomorrow. We also did a PCR to test the ligations. | |
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| - | + | We miniprepped the ligations we streaked out yesterday and part [http://partsregistry.org/Part:BBa_R1052 R1052] and [http://partsregistry.org/Part:BBa_R0040 R0040]. It's the second time we miniprep R1052 and the concentrations was two times very low. The registry shows that there are a few problems with this part. | |
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| - | + | We made some digests: [http://partsregistry.org/Part:BBa_C0056 C0056] and [http://partsregistry.org/Part:BBa_C0061 C0061] with ''Spe''I and ''Pst''I and [http://partsregistry.org/Part:BBa_R0040 R0040] with ''Spe''I and ''Eco''RI. We couldn't see R0040 on gel and C0056 seemed to be too big, only C0061 was ok. We purified C0061 and C0056. | |
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| + | We made a ligation of [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] (cut with ''Pst''I instead of ''Eco''RI). | ||
=== Dry Lab === | === Dry Lab === | ||
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==== Wiki ==== | ==== Wiki ==== | ||
Components section has been updated further. Also the modeling section is getting some new lines and graphics ... | Components section has been updated further. Also the modeling section is getting some new lines and graphics ... | ||
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Latest revision as of 13:06, 16 October 2008
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
We did a lot of PCRs today. We tried to add the second part of the UmuD tag with the 91bp-primer. We had very sharp and extremely beautiful bands, but they seemed three times too long: FAIL. This will be done again tomorrow, with primers 2.1 and 2.2. The antisense LuxI PCR resulted in a smear: FAIL again. To be done again tomorrow. We also did a PCR to test the ligations.
We miniprepped the ligations we streaked out yesterday and part [http://partsregistry.org/Part:BBa_R1052 R1052] and [http://partsregistry.org/Part:BBa_R0040 R0040]. It's the second time we miniprep R1052 and the concentrations was two times very low. The registry shows that there are a few problems with this part.
We made some digests: [http://partsregistry.org/Part:BBa_C0056 C0056] and [http://partsregistry.org/Part:BBa_C0061 C0061] with SpeI and PstI and [http://partsregistry.org/Part:BBa_R0040 R0040] with SpeI and EcoRI. We couldn't see R0040 on gel and C0056 seemed to be too big, only C0061 was ok. We purified C0061 and C0056.
We made a ligation of [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] (cut with PstI instead of EcoRI).
Dry Lab
Ethics...
Modeling
Latex-tool... Multi-cell...
Wiki
Components section has been updated further. Also the modeling section is getting some new lines and graphics ...
