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Team:UC Berkeley/LysisDevice
From 2008.igem.org
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The purpose of the lysis device is to allow for the easy release of any product produced by a Clonebot device. | The purpose of the lysis device is to allow for the easy release of any product produced by a Clonebot device. | ||
Revision as of 23:00, 19 October 2008
Introduction
The purpose of the lysis device is to allow for the easy release of any product produced by a Clonebot device.
Device
One of our lysis devices is based on the lambda phage, and the other on the T4 phage. Lysis is caused by the combined action of a holin protein and lysozyme - the holin creates pores in the bacterial inner membrane, which allows the lysozyme to reach the periplasm and break down the peptidoglycans. In both systems, antiholin inhibits holin and prevents the creation of pores. In our lysis devices, a constituitive promoter drives the production of antiholin, while an inducible promoter drives the production of holin and lysozyme.
