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Team:Hawaii/Notebook/2008-10-21
From 2008.igem.org
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(→Insertion of lac-GFP device into BB-pRL1383a) |
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:* Made LB+sp<sub>variable</sub> plates with 40 μl 20X X-gal | :* Made LB+sp<sub>variable</sub> plates with 40 μl 20X X-gal | ||
:* Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a (4 ng) | :* Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a (4 ng) | ||
| - | :* Plated 50 & | + | :* Plated 50 μl cells + SOB on each plate |
:* Incubated at 37C for 2 days | :* Incubated at 37C for 2 days | ||
Revision as of 23:23, 21 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Insertion of lac-GFP device into BB-pRL1383a
- Grace
- Ran RE digests on gel
- Bands were all incorrect size
- Reinoculated TB+sp100 for plasmid prep tomorrow
- PCR of J04430 from filter paper to verify part; used H/B primers
Antibiotic test for BB-pRL1383a
- Grace
- Made LB+spvariable plates with 40 μl 20X X-gal
- Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a (4 ng)
- Plated 50 μl cells + SOB on each plate
- Incubated at 37C for 2 days
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
