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Team:Hawaii/Notebook/2008-10-22
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BB-pRL1383a=== :<strong> Grace</strong> :* ''E. coli'' did not grow. Reinoculated. ===Extracted J0443...) |
(→Extracted J04430 from filter paper) |
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[[Image:102208J04430.png|right|thumb|200px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded.]] | [[Image:102208J04430.png|right|thumb|200px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded.]] | ||
:* Used 2 μl of filter paper solution for PCR to verify plasmid | :* Used 2 μl of filter paper solution for PCR to verify plasmid | ||
| + | ::* Something is wrong with the J04430 filter spot iGEM sent us. PCR with VF2/VR primers did NOT yield the expected band ~1000bp. | ||
===Transformation=== | ===Transformation=== | ||
Revision as of 22:12, 22 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep of BB-pRL1383a
- Grace
- E. coli did not grow. Reinoculated.
Extracted J04430 from filter paper
- Grace
- Used 2 μl of filter paper solution for PCR to verify plasmid
- Something is wrong with the J04430 filter spot iGEM sent us. PCR with VF2/VR primers did NOT yield the expected band ~1000bp.
Transformation
- Grace
- Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
