Team:Hawaii/Notebook/2008-10-21

From 2008.igem.org

(Difference between revisions)
(Antibiotic test for BB-pRL1383a)
(Insertion of lac-GFP device into BB-pRL1383a)
Line 5: Line 5:
===Insertion of lac-GFP device into BB-pRL1383a===
===Insertion of lac-GFP device into BB-pRL1383a===
:<strong>  Grace</strong>
:<strong>  Grace</strong>
-
[[Image:102108REdigests.png|right|thumb|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]]
+
[[Image:102108REdigests.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]][[Image:102108J04430.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.]]
:*Ran RE digests on gel
:*Ran RE digests on gel
::*Bands were all incorrect size
::*Bands were all incorrect size

Revision as of 08:50, 23 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Insertion of lac-GFP device into BB-pRL1383a

Grace
EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.
EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.
  • Ran RE digests on gel
  • Bands were all incorrect size
  • Reinoculated TB+sp100 for plasmid prep tomorrow
  • PCR of J04430 from filter paper to verify part; used H/B primers

Team:Hawaii/Antibiotic test for BB-pRL1383a

Grace
  • Made LB+spvariable plates with 50 μl 20X X-gal
  • Transformed 100 μl DH5α with 2 μl BB-pRL1383a (4 ng)
  • Plated 50 μl cells + SOB on each LB+sp plate; plated 20 μl untransformed cells + SOB on each control plate
  • Incubated at 37C for 2 days

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]