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Team:Heidelberg/Notebook/Sensing Group/Notebook/2ndweek
From 2008.igem.org
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== Thursday, 08/14/2008 == | == Thursday, 08/14/2008 == | ||
[[Image:HD 080814-LuxQ PCR.png|thumb|right|PCR for LuxQ and LuxQ-2]] | [[Image:HD 080814-LuxQ PCR.png|thumb|right|PCR for LuxQ and LuxQ-2]] | ||
| - | * Gel from LuxQ, | + | * Gel from LuxQ, LuxQ2 |
* sequentiell digestion of Fusion-1 with NcoI and NdeI (NEBuffer 3) | * sequentiell digestion of Fusion-1 with NcoI and NdeI (NEBuffer 3) | ||
* Double digestion of pDK48 with NcoI/NdeI (NEBuffer 4) | * Double digestion of pDK48 with NcoI/NdeI (NEBuffer 4) | ||
Revision as of 12:47, 26 October 2008
Contents |
Monday, 08/11/2008
- preparation of LuxS harboring cells O/N culture
Tuesday, 08/12/2008
- Miniprep of correctly sequenced LuxS and Glycerol-stock
- LuxQ no. 3 checked via digestion with NcoI/BamHI --> negative result
- PCR for LuxQ, LuxQ-1, LuxQ-2, Tar-1, Tar-2 with Taq Mastermix
- preparation of O/N culture for LuxQ no. 3
Wednesday, 08/13/2008
- PCR-purification of the 5 PCR-products eluted in 50 µl H2O
- Miniprep of LuxQ no. 3 O/N culture and digestion with xbaI
- Fusion-PCR for Fusion-1 and repetetion of PCR for LuxQ-2 and LuxQ
- digestion of pDK48 and Fusion-1 with NcoI/NdeI (0.5 µl) and AgeI (1 µl) in NEBuffer 4
Thursday, 08/14/2008
- Gel from LuxQ, LuxQ2
- sequentiell digestion of Fusion-1 with NcoI and NdeI (NEBuffer 3)
- Double digestion of pDK48 with NcoI/NdeI (NEBuffer 4)
