Team:KULeuven/Parts

From 2008.igem.org

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{{:Team:KULeuven/Tools/Scripting}}
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== Submitted Parts ==
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{{:Team:KULeuven/Tools/Header}}
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{| style="background:#99CCFF;color:black;width:100%;" border="0" cellpadding="10" cellspacing="0" align="center"
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|-
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! Part number
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! Replaced by
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! Description
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! Type
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! Component
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|- style="background:#f0f0f0; color:black"
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| '''K145000'''
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| '''K145100'''
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| eCFP with LVA tag under OmpR control
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| part
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| input
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|- style="background:#f0f0f0; color:black"
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| '''K145001'''
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| /
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| T7 RNA polymerase
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| part
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| filter plasmid 2
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|- style="background:#f0f0f0; color:black"
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| '''K145002'''
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| /
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| T7 RNA Polymerase with LVA tag under OmpF control
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| composite part
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| filter plasmid 1
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|- style="background:#f0f0f0; color:black"
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| '''K145003'''
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| '''K145102'''
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| RiboKey 3d under control of T7 RNA polymerase promotor
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| /
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| /
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|- style="background:#f0f0f0; color:black"
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| '''K145004'''
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| '''K145104'''
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|  T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.
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| /
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| /
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|- style="background:#f0f0f0; color:black"
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| '''K145005'''
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| /
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| cI under control of a dual promotor and a RiboLock.
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|- style="background:#f0f0f0; color:black"
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| '''K145006'''
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| '''K145106'''
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| Lactonase under PRM control
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|- style="background:#f0f0f0; color:black"
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| '''K145007'''
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| '''K145107'''
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| Pulse Generator
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|- style="background:#f0f0f0; color:black"
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| '''K145008'''
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| '''K145108'''
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| Constitutive production of LuxR
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|- style="background:#f0f0f0; color:black"
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| '''K145009'''
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| '''K145109'''
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| ccdB cell death gene under control of an activating Lux PR
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|
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|- style="background:#f0f0f0; color:black"
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| '''K145010'''
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| '''K145110'''
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| Killing switch
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|- style="background:#f0f0f0; color:black"
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| '''K145011'''
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| /
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| Light sensor to provide dummy input for the system
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|
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| input
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|- style="background:#f0f0f0; color:black"
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| '''K145012'''
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| /
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| N-terminal degradation tag
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| filter
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|- style="background:#f0f0f0; color:black"
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| '''K145013'''
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| /
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| antisense LuxI
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|- style="background:#f0f0f0; color:black"
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| '''K145100'''
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| /
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| eCFP with LVA tag under control of a OmpF promotor
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|- style="background:#f0f0f0; color:black"
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| '''K145101'''
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| /
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| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor.
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|- style="background:#f0f0f0; color:black"
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| '''K145102'''
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| /
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| RiboKey3d under control of OmpF promotor
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|
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|filter
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|- style="background:#f0f0f0; color:black"
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| '''K145103'''
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| /
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| T7 with an LVA tag under control of a constitutive promotor and a RiboLock3d
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|- style="background:#f0f0f0; color:black"
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| '''K145104'''
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| /
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| AND part, T7 and RiboKey3d together will produce LacI. This will repress transcription of LuxI from a pLac promotor
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| inverter
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|- style="background:#f0f0f0; color:black"
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| '''K145105'''
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| /
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
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|- style="background:#f0f0f0; color:black"
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| '''K145106'''
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| Lactonase under PRM control
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|- style="background:#f0f0f0; color:black"
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| '''K145107'''
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| '''K145117'''
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| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
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|- style="background:#f0f0f0; color:black"
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| '''K145108'''
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| LuxR and TetR under control of a constitutive promotor.
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|- style="background:#f0f0f0; color:black"
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| '''K145109'''
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| ccdB cell death gene under the control of a LuxPR promotor.
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|- style="background:#f0f0f0; color:black"
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| '''K145110'''
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| Complete cell death mechanism. Combination of '''BBa_ K145108''' and '''BBa_K145109'''.
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|- style="background:#f0f0f0; color:black"
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| '''K145111'''
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| /
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| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor
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|- style="background:#f0f0f0; color:black"
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| '''K145112'''
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
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|- style="background:#f0f0f0; color:black"
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| '''K145115'''
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
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|- style="background:#f0f0f0; color:black"
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| '''K145117'''
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| /
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| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
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|}
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==The Big Sheme of Used Parts==
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[https://static.igem.org/mediawiki/2008/6/6d/THE_BIG_SCHEME.xls The Big Scheme]
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Latest revision as of 00:10, 27 October 2008

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