Brown: Team Resistance/27 October 2008

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*Another miniprep of the S105 gene transformation performed.
*Another miniprep of the S105 gene transformation performed.
*Optical Density Measurement: 71.6 ng/ul
*Optical Density Measurement: 71.6 ng/ul
 +
*Restriction Digest Verification done of S105 gene with restriction enzymes EcoRI and PstI (Buffer H). Placed in thermocycler for 1 hour at 37 degrees and heat shock 20 minutes at 80 deg C.
*Performed another ligation of PVJ4 and pSB1AK3.  We are still seeing no growth on the PVJ4 or S105 cassette plates.
*Performed another ligation of PVJ4 and pSB1AK3.  We are still seeing no growth on the PVJ4 or S105 cassette plates.

Revision as of 14:32, 27 October 2008


Toxipop lab notebook.png

October 27, 2008

  • Another miniprep of the S105 gene transformation performed.
  • Optical Density Measurement: 71.6 ng/ul
  • Restriction Digest Verification done of S105 gene with restriction enzymes EcoRI and PstI (Buffer H). Placed in thermocycler for 1 hour at 37 degrees and heat shock 20 minutes at 80 deg C.


  • Performed another ligation of PVJ4 and pSB1AK3. We are still seeing no growth on the PVJ4 or S105 cassette plates.
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