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Team:Freiburg Cloning Strategy
From 2008.igem.org
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| + | [[Image:Freiburg2008_TV_CMVRluc.jpg|700px]] | ||
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| + | '''Figure 1''' A shows the Biobrick part BBa_J52017. The transfectionvektor for eukaryotic cell systems has an ampicillin- and a kanamycin resistance cassette. The multiple cloning site contains the Biobrick standard restriction sites EcoRI, NotI, XbaI, SpeI, NotI, PstI followed by an eukaryotic terminator sequence. B The CMV-promotor fragment was obtained by PCR with the Biobrick BBa-J52038 template. C The PCR product was cloned into the transfectionvector by EcoRI and PstI to get a final eukaryotic transfection-system. D To test the efficiency of expression a gene-fragment coding for the yellow fluorescent protein was put into the vector behind CMV-Promotor.<br> | ||
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[[Image:Freiburg2008_Konstrukte.jpg|700px]] | [[Image:Freiburg2008_Konstrukte.jpg|700px]] | ||
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[[Image:Freiburg2008_SP_LIPO_GGGS_TM_bla1_YFP_2.jpg|700px]] | [[Image:Freiburg2008_SP_LIPO_GGGS_TM_bla1_YFP_2.jpg|700px]] | ||
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[[Image:Freiburg2008_TV_CMV_YFP___CFP_loeslich.jpg|700px]] | [[Image:Freiburg2008_TV_CMV_YFP___CFP_loeslich.jpg|700px]] | ||
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Revision as of 08:53, 28 October 2008
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_cloning strategy
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