Team:Princeton/Experiments

From 2008.igem.org

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3. [[PCR SOEing]]
3. [[PCR SOEing]]
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4. [[Run Gel]]
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4. [[Run Gel/ Gel extraction]]
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5. [[Gel Extraction]]
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5. [[Digestion]]
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6. [[Digestion]]
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6. [[CIP Treatment]]
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8. [[CIP Treatment]]
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7. [[PCR Purification]]
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9. [[PCR Purification]]
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8. [[Ligation]]
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10. [[Ligation]]
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9. [[Transformation and Plating]]
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11. [[Transformation and Plating]]
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10. [[Extract DNA (Miniprep)]]
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12. [[Extract DNA (Miniprep)]]
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11. [[Restriction Map/ Digest]]
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13. [[Restriction Map/ Digest]]
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12. [[Re-transform with selected plasmid]]
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14. [[Re-transform with selected plasmid]]
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13. [[Extract DNA (Maxiprep or Midiprep)]]
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15. [[Extract DNA (Maxiprep or Midiprep)]]
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14. [[Sequence]]
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16. [[Sequence]]
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===Bio-Brick Verification===
===Bio-Brick Verification===

Revision as of 17:17, 28 October 2008

PrincetonLogo.gif

PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. PCR SOEing

4. Run Gel/ Gel extraction

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligation

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence

Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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