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Team:ESBS-Strasbourg/Protocols
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| - | == | + | <div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div> |
| + | <div id="maincontent" style="margin-top:150px;"> | ||
| - | + | __NOTOC__ | |
| - | + | =Protocols= | |
| + | <br> | ||
| + | {| | ||
| + | |-valign="top" | ||
| + | |width="360px"| | ||
| + | ==E.Coli== | ||
| - | + | *[[Team:ESBS-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br> | |
| + | *[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)] <br> | ||
| + | *[[Team:ESBS-Strasbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br> | ||
| + | *[[Team:ESBS-Strasbourg/Transformation Heat shock|Heat shock transformation]]<br> | ||
| + | |||
| + | |width="360px"| | ||
==Cloning== | ==Cloning== | ||
| - | [[PCR]] <br> | + | *[[Team:ESBS-Strasbourg/PCR|PCR]] <br> |
| - | [[Purification of PCR product]] <br> | + | *[[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR products]] <br> |
| - | [[Miniprep: plasmid amplification]] <br> | + | *[[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:Plasmid Amplification]] <br> |
| - | [[Plasmid Purification]] <br> | + | *[[Team:ESBS-Strasbourg/Plasmid urification|Plasmid Purification]] <br> |
| - | [[Restriction digestion]] <br> | + | *[[Team:ESBS-Strasbourg/Restriction digestion|Restriction Digestion]] <br> |
| - | [[Agarose gel preparation]] <br> | + | *[[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose Gel Preparation]] <br> |
| - | [[Dephosphorylation]] <br> | + | *[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> |
| - | [[Ligation]] <br> | + | *[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> |
| + | *[[Team:ESBS-Strasbourg/Concentrations Measurements|Concentrations Measurements]] <br> | ||
| + | |width="360px"| | ||
==Solutions and buffers== | ==Solutions and buffers== | ||
| - | + | *[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells] <br> | |
| + | *[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes] <br> | ||
| + | *[[Team:ESBS-Strasbourg/TENS|TENS for the do it yourself MiniPrep]] <br> | ||
| + | |} | ||
| + | <br> | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org] | ||
| + | |||
| + | <br> | ||
Latest revision as of 00:13, 29 October 2008
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Protocols
E.Coli
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Cloning |
Solutions and buffers
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Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]
