Team:ESBS-Strasbourg/Protocols

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==E.Coli==
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<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
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<div id="maincontent" style="margin-top:150px;">
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'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
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__NOTOC__
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'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
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=Protocols=
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<br>
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{|
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|-valign="top"
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|width="360px"|
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==E.Coli==
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'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br>
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*[[Team:ESBS-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br>
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*[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)] <br>
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*[[Team:ESBS-Strasbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br>
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*[[Team:ESBS-Strasbourg/Transformation Heat shock|Heat shock transformation]]<br>
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|width="360px"|
==Cloning==
==Cloning==
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[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
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*[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
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[[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR product]] <br>
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*[[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR products]] <br>
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[[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:plasmid amplification]] <br>
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*[[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:Plasmid Amplification]] <br>
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[[Team:ESBS-Strasbourg/Plasmid urification|Plasmid purification]] <br>
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*[[Team:ESBS-Strasbourg/Plasmid urification|Plasmid Purification]] <br>
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[[Team:ESBS-Strasbourg/Restriction digestion|Restriction digestion]] <br>
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*[[Team:ESBS-Strasbourg/Restriction digestion|Restriction Digestion]] <br>
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[[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose gel preparation]] <br>
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*[[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose Gel Preparation]] <br>
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[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br>
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*[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br>
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[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
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*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
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*[[Team:ESBS-Strasbourg/Concentrations Measurements|Concentrations Measurements]] <br>
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|width="360px"|
==Solutions and buffers==
==Solutions and buffers==
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'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
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*[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells] <br>
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*[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes] <br>
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*[[Team:ESBS-Strasbourg/TENS|TENS for the do it yourself MiniPrep]] <br>
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|}
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<br>
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----
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Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]
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<br>

Latest revision as of 00:13, 29 October 2008


Protocols


E.Coli

Cloning

Solutions and buffers

  • [http://openwetware.org/wiki/SOB SOB for the preparation of competent cells]
  • [http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes]
  • TENS for the do it yourself MiniPrep



Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]


Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/Protocols"