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Brown: Team Resistance/24 June 2008
From 2008.igem.org
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*Apparatus construction: Lab View and Data Acquisition testing. | *Apparatus construction: Lab View and Data Acquisition testing. | ||
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| + | Trip to Radioshack: | ||
| + | *resistors → 1Mohm and 10Mohm | ||
| + | *22 gauge solid core copper hook-up wire | ||
| + | *wire crimpers | ||
| + | *gold plated terminals | ||
| + | |||
| + | Practice Set-up | ||
| + | |||
| + | Daniel Ludwig came to our lab and helped us create our circuit and use LabView | ||
| + | *First measure actual resistance of purchased resistors with multimeter: | ||
| + | 1) 0.988Mohm→ this becomes our comparison resistor | ||
| + | *Put resistor in breadboard to PCI: | ||
| + | *Channel: AI1, Pin #: 33 | ||
| + | *Channel: AI GND, Pin #: 67 | ||
| + | **Used apparatus to measure resistance of a 10Mohm resistor | ||
| + | *AO1, pin#21 | ||
| + | *AO GND, pin#55 | ||
| + | **Measured resistance of a 10Mohm resistor with apparatus | ||
| + | *Resistance measured by multimeter: 2) 9.86Mohm | ||
| + | *Our set-up led to fluctuating and inaccurate resistance measurement at first | ||
| + | *Daniel Ludwig’s email said that LabView has a 30 minute warm-up period | ||
| + | *When experiment tried again, resistance of 9.84-9.85Mohm was recorded by our apparatus =) | ||
| + | *Real-time measurements possible | ||
| + | *Graph can be created directly in LabView (Aaron said he could help us) | ||
| + | |||
| + | Made 3, 2mL cultures of bacteria from Harvard plate 75 (pVJ13) to compare its growth to the pVJ4 because growth has appeared slow —incubator 8:10pm-- in LB with NO ANTIBIOTIC to see if growth increases | ||
| + | |||
| + | Requested MDL for use of their spec to check whether our Nanodrop is giving inaccurate readings | ||
| + | |||
| + | Tomorrow: | ||
| + | *Meeting with Dr. Jill Kreiling 2:30pm | ||
| + | *Run experiments with saltwater to test efficiency of apparatus | ||
| + | *Check growth of cell cultures | ||
Latest revision as of 00:27, 29 October 2008
24 June 2008
Trip to Radioshack:
Practice Set-up Daniel Ludwig came to our lab and helped us create our circuit and use LabView
1) 0.988Mohm→ this becomes our comparison resistor
Made 3, 2mL cultures of bacteria from Harvard plate 75 (pVJ13) to compare its growth to the pVJ4 because growth has appeared slow —incubator 8:10pm-- in LB with NO ANTIBIOTIC to see if growth increases Requested MDL for use of their spec to check whether our Nanodrop is giving inaccurate readings Tomorrow:
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