Team:Princeton/Experiments

From 2008.igem.org

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{{PrincetonHeader}}
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===Bio-brick Creation===
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==Bio-brick Creation==
'''Lab Protocols'''
'''Lab Protocols'''
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{|
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|1. Primer Design
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1. Primer Design
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2. [[PCR Amplification]]
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2. PCR
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3. [[Run Gel/ Gel extraction]]
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3. Run Gel
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4. [[PCR SOEing]]
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4. Gel Extraction
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5. [[Digestion]]
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5. Digestion
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6. [[CIP Treatment]]
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6. CIP Treatment
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7. [[PCR Purification]]
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7. PCR Purification
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|8. [[Ligate DNA]]
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8. Ligation
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9. [[Transformation and Plating]]
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9. Transformation and Plating
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10. [[Extract DNA (Miniprep)]]
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10. Extract DNA (Miniprep)
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11. [[Restriction Map/ Restriction Digest]]
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11. Restriction Map/ Digest
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12. [[Re-transform with selected plasmid]]
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12. Re-transform with selected plasmid
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13. [[Extract DNA (Maxiprep or Midiprep)]]
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13. Extract DNA (Maxiprep or Midiprep)
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14. [[Sequence]]
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|}
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14. Sequence
 
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==Bio-Brick Verification==
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'''Cell Culture Process'''
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===Bio-Brick Verification===
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1. [[Lentivirus Production]]
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'''Cell Culture'''
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2. [[Lentivirus Harvesting]]
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3. [[Infection]]
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Also,
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1. [[Transfection (nonviral)]]

Latest revision as of 07:57, 29 October 2008

PrincetonLogo.gif

PRINCETON IGEM 2008

Home Project Overview Project Details Experiments Results Notebook
Parts Submitted to the Registry Modeling The Team Gallery




Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. Run Gel/ Gel extraction

4. PCR SOEing

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligate DNA

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Restriction Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence


Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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