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Team:Princeton/Experiments
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| - | + | ==Bio-brick Creation== | |
'''Lab Protocols''' | '''Lab Protocols''' | ||
| + | {| | ||
| + | |1. Primer Design | ||
| - | + | 2. [[PCR Amplification]] | |
| - | + | 3. [[Run Gel/ Gel extraction]] | |
| - | + | 4. [[PCR SOEing]] | |
| - | + | 5. [[Digestion]] | |
| - | + | 6. [[CIP Treatment]] | |
| - | + | 7. [[PCR Purification]] | |
| - | + | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| | |
| + | |8. [[Ligate DNA]] | ||
| - | + | 9. [[Transformation and Plating]] | |
| - | + | 10. [[Extract DNA (Miniprep)]] | |
| - | + | 11. [[Restriction Map/ Restriction Digest]] | |
| - | + | 12. [[Re-transform with selected plasmid]] | |
| - | + | 13. [[Extract DNA (Maxiprep or Midiprep)]] | |
| - | + | 14. [[Sequence]] | |
| + | |} | ||
| - | |||
| - | + | ==Bio-Brick Verification== | |
'''Cell Culture Process''' | '''Cell Culture Process''' | ||
| - | 1. Lentivirus Production | + | 1. [[Lentivirus Production]] |
| - | 2. Lentivirus Harvesting | + | 2. [[Lentivirus Harvesting]] |
| - | 3. Infection | + | 3. [[Infection]] |
| Line 47: | Line 50: | ||
Also, | Also, | ||
| - | 1. Transfection (nonviral) | + | 1. [[Transfection (nonviral)]] |
Latest revision as of 07:57, 29 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
|---|
| Parts Submitted to the Registry | Modeling | The Team | Gallery |
|---|
Bio-brick Creation
Lab Protocols
| 1. Primer Design
4. PCR SOEing 5. Digestion | 8. Ligate DNA
11. Restriction Map/ Restriction Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence |
Bio-Brick Verification
Cell Culture Process
3. Infection
Also,
