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Team:Princeton/Experiments
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| - | + | ==Bio-brick Creation== | |
'''Lab Protocols''' | '''Lab Protocols''' | ||
| - | + | {| | |
| - | 1. | + | |1. Primer Design |
2. [[PCR Amplification]] | 2. [[PCR Amplification]] | ||
| - | 3. [[ | + | 3. [[Run Gel/ Gel extraction]] |
| - | 4. [[ | + | 4. [[PCR SOEing]] |
5. [[Digestion]] | 5. [[Digestion]] | ||
| Line 19: | Line 19: | ||
7. [[PCR Purification]] | 7. [[PCR Purification]] | ||
| - | 8. [[ | + | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
| + | |8. [[Ligate DNA]] | ||
9. [[Transformation and Plating]] | 9. [[Transformation and Plating]] | ||
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10. [[Extract DNA (Miniprep)]] | 10. [[Extract DNA (Miniprep)]] | ||
| - | 11. [[Restriction Map/ Digest]] | + | 11. [[Restriction Map/ Restriction Digest]] |
12. [[Re-transform with selected plasmid]] | 12. [[Re-transform with selected plasmid]] | ||
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14. [[Sequence]] | 14. [[Sequence]] | ||
| + | |} | ||
| + | |||
| - | + | ==Bio-Brick Verification== | |
'''Cell Culture Process''' | '''Cell Culture Process''' | ||
Latest revision as of 07:57, 29 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
|---|
| Parts Submitted to the Registry | Modeling | The Team | Gallery |
|---|
Bio-brick Creation
Lab Protocols
| 1. Primer Design
4. PCR SOEing 5. Digestion | 8. Ligate DNA
11. Restriction Map/ Restriction Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence |
Bio-Brick Verification
Cell Culture Process
3. Infection
Also,
