Team:Paris/Modeling/f0

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(Difference between revisions)
Line 24: Line 24:
|dilution-degradation rate <br> of GFP(mut3b)
|dilution-degradation rate <br> of GFP(mut3b)
|min<sup>-1</sup>
|min<sup>-1</sup>
-
|
+
|0.0198
 +
|Only dilution :<br>Time Cell Division : 35 min.
|-
|-
|[GFP]
|[GFP]

Revision as of 08:42, 29 October 2008

Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

param signification unit value comments
[exprJ23101] expression rate of
J23101 with RBS E0032 		nM.min-1
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration 		nM.au-1 (1/79.429)

That will directly give us ƒ0

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