Team:Harvard/Dailybook/Week5/Widgetry

From 2008.igem.org

(Difference between revisions)
(New page: =Monday: July 21, 2008= *Lab meeting *Cleaned chambers =Tuesday: July 22, 2008= *started mtrB and wt cultures *troubleshoot LabVIEW =Wednesday: July 23, 2008= =Thursday: July 24, 2008= =...)
(Monday: July 21, 2008)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
-
=Monday: July 21, 2008=
+
= Goals for the Week =
-
*Lab meeting
+
* Last week, had experiment with no current at all
-
*Cleaned chambers
+
** Hypothesized that cells were dead?
 +
** Run test with 1 day vs. 2 day old Shewie to determine if there is an effect
 +
 
 +
 
=Tuesday: July 22, 2008=
=Tuesday: July 22, 2008=
*started mtrB and wt cultures
*started mtrB and wt cultures
 +
** mtrB culture to be used as 2-day culture on Thursday
*troubleshoot LabVIEW
*troubleshoot LabVIEW
 +
** Resistances were measured to be different as expected in the past experiment
 +
** Some as low as 160 ohms
 +
** Perhaps the resistance of the chambers are less than we expected?
=Wednesday: July 23, 2008=
=Wednesday: July 23, 2008=
 +
* Started another mtrB culture
 +
** To be used as 1 day culture in experiment on Thursday
 +
* LabVIEW
 +
** Determined that resistance should be measured before connecting to the fuel cells
 +
** Resistance of chambers is the same as what we measured initially
 +
=Thursday: July 24, 2008=
=Thursday: July 24, 2008=
 +
* mtrB 1 day vs. 2 day experiment
 +
** 6 chambers
 +
*** 3 chambers with 1-day old mtrB
 +
*** 3 chambers with 2-day old mtrB
 +
*** Injected lactate after the initial current spike came down
 +
** Hypothesis
 +
***2-day cultures will have lower (if any) current production
 +
=Friday: July 25, 2008=
=Friday: July 25, 2008=
 +
[[Image:mtrb_1dv2d.jpg | 600px]]
 +
 +
'''mtrB: Overnight versus 2 Day Liquid Cultures'''
 +
 +
 +
What we did:
 +
 +
-Ran 1-day old mtrB versus 2-day old mtrB
 +
 +
 +
Results:
 +
 +
-The shapes of the curves were all very similar.
 +
 +
-Overnight cultures had slightly higher current production than 2-day old cultures.

Latest revision as of 19:22, 29 October 2008

Contents

Goals for the Week

  • Last week, had experiment with no current at all
    • Hypothesized that cells were dead?
    • Run test with 1 day vs. 2 day old Shewie to determine if there is an effect


Tuesday: July 22, 2008

  • started mtrB and wt cultures
    • mtrB culture to be used as 2-day culture on Thursday
  • troubleshoot LabVIEW
    • Resistances were measured to be different as expected in the past experiment
    • Some as low as 160 ohms
    • Perhaps the resistance of the chambers are less than we expected?

Wednesday: July 23, 2008

  • Started another mtrB culture
    • To be used as 1 day culture in experiment on Thursday
  • LabVIEW
    • Determined that resistance should be measured before connecting to the fuel cells
    • Resistance of chambers is the same as what we measured initially

Thursday: July 24, 2008

  • mtrB 1 day vs. 2 day experiment
    • 6 chambers
      • 3 chambers with 1-day old mtrB
      • 3 chambers with 2-day old mtrB
      • Injected lactate after the initial current spike came down
    • Hypothesis
      • 2-day cultures will have lower (if any) current production

Friday: July 25, 2008

Mtrb 1dv2d.jpg

mtrB: Overnight versus 2 Day Liquid Cultures


What we did:

-Ran 1-day old mtrB versus 2-day old mtrB


Results:

-The shapes of the curves were all very similar.

-Overnight cultures had slightly higher current production than 2-day old cultures.