Team:Chiba/Calendar-Home/29 August 2008

From 2008.igem.org

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'''OUTPUT TEAM'''
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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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*time response measurement(plate)
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 +
[[Team:Chiba/Calendar-Home/28 August 2008|28 August 2008 <]]|[[Team:Chiba/Calendar-Home/30 August 2008|> 30 August 2008]]
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 +
==Laboratory work==
 +
===Team:Input===
 +
no work
 +
 
 +
===Team:Communication===
 +
 
 +
:--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]'''
 +
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
 +
::#[http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
 +
::#[http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
 +
::#[http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
 +
::#[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
 +
::#[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
 +
::#[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
 +
::#[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
 +
::#[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
 +
::#[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2006)
 +
 
 +
 
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>S1~10</td><td>W1</td><td>W2~10</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
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<td>1</td><td>4</td><td>2</td>
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</tr>
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<tr>
 +
<td>PstI</td>
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<td>-</td><td>0.075</td><td>0.1</td>
 +
</tr>
 +
<tr>
 +
<td>EcoRI</td>
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<td>0.1</td><td>0.075</td><td>0.1</td>
 +
</tr>
 +
<tr>
 +
<td>Buffer 3</td>
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<td>1</td><td>0.75</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>BSA</td>
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<td>-</td><td>0.75</td><td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>7.9</td><td>4.35</td><td>5.8</td>
 +
</tr>
 +
<tr>
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<td>TOTAL</td>
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<td>10μl</td><td>10μl</td><td>10μl</td>
 +
</tr>
 +
</table>
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0829.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
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<td>1~10</td><td>S1~S10,W1~W10</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
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<td>1</td><td>10</td>
 +
</tr>
 +
<tr>
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<td>Loading Dye</td>
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<td>1</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>6μl</td><td>12μl</td></tr>
 +
</table>
 +
:From left;<br>
 +
::1~5,S1,S5,W1~W5
 +
|}
 +
{|align="justify"
 +
|[[Image:Chiba-0829-2.JPG]]
 +
|
 +
:From left;<br>
 +
::6~10,S6~S10,W6~W10
 +
|}
 +
 
 +
 
 +
 
 +
:(27/8)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0829-3.JPG]]
 +
|
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
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<td>0.5</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
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<td>4.5</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>6μL</td></tr>
 +
</table>
 +
 
 +
::[http://partregistry.org/Part:R0010 R0010] -> OK
 +
|}
 +
 
 +
 
 +
'''[[Team:Chiba/protocol/digestion|Digestion]]'''
 +
::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
 +
::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
 +
::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
 +
::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2006)
 +
 
 +
 
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
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<td>1~3</td><td>4</td>
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</tr>
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<tr>
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<td>Sample DNA</td>
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<td>100ng/μL×20</td><td>200ng/μL×30</td>
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</tr>
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<tr>
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<td>PstI</td>
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<td>2</td><td>2</td>
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</tr>
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<tr>
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<td>XbaI</td>
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<td>2</td><td>-</td>
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</tr>
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<tr>
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<td>SpeI</td>
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<td>-</td><td>2</td></tr>
 +
<tr>
 +
<td>Buffer 2</td>
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<td>-</td><td>5</td></tr>
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<tr>
 +
<tr>
 +
<td>Buffer 3</td>
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<td>5</td><td>-</td></tr>
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<tr>
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<td>BSA</td>
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<td>5</td><td>5</td>
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</tr>
 +
<tr>
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<td>dH<sub>2</sub>O</td>
 +
<td>16</td><td>6</td></tr>
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<tr>
 +
<td>TOTAL</td>
 +
<td>50μl</td><td>50μl</td>
 +
</tr>
 +
</table>
 +
 
 +
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0829-4.JPG]]
 +
|
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>1~3</td><td>4</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
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<td>10</td><td>3</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
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<td>2</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>-</td><td>7</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12μl</td><td>12μl</td></tr>
 +
</table>
 +
 
 +
:insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026] : 685bp -> None<br>
 +
|}
 +
{|align="justify"
 +
|[[Image:Chiba-0829-5.JPG]]
 +
|
 +
:insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026]  : 745bp -> None<br>
 +
|}
 +
{|align="justify"
 +
|[[Image:Chiba-0829-6.JPG]]
 +
|
 +
:insert[http://partsregistry.org/BBa_S03154 BBa_S03154] : 685bp -> None<br>
 +
|}
 +
{|align="justify"
 +
|[[Image:Chiba-0829-7.JPG]]
 +
|
 +
:vector[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] : 2079bp -> OK<br>
 +
|}
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
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:::->15μL
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 +
:--->'''[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]'''
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No.</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
 +
<td>15</td>
 +
</tr>
 +
<tr>
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<td>SAP</td>
 +
<td>2</td></tr>
 +
<tr>
 +
<td>SAP Buffer</td>
 +
<td>3</td></tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>10</td></tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>30μL</td></tr>
 +
</table>
 +
 
 +
 
 +
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
 +
 
 +
::--->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)
 +
 
 +
===Team:Output===
 +
[[Team:Chiba/jk/γ/trs|TIME RESPONCE(solid)]]

Latest revision as of 20:28, 29 October 2008

>Home | Notebook

28 August 2008 <|> 30 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

--->Digestion Test
  1. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
  2. [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
  3. [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
  4. [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
  5. [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
  6. [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
  7. [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
  8. [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
  9. [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
  10. [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2006)


Sample No. S1~10W1W2~10
Sample DNA 142
PstI -0.0750.1
EcoRI 0.10.0750.1
Buffer 3 10.751
BSA -0.751
dH2O 7.94.355.8
TOTAL 10μl10μl10μl


--->Gel Check
Chiba-0829.JPG
Sample No. 1~10S1~S10,W1~W10
Sample DNA 110
Loading Dye 12
TOTAL 6μl12μl
From left;
1~5,S1,S5,W1~W5
Chiba-0829-2.JPG
From left;
6~10,S6~S10,W6~W10


(27/8)--->Gel Check
Chiba-0829-3.JPG
Sample DNA 0.5
Loading Dye 1
dH2O 4.5
TOTAL 6μL
[http://partregistry.org/Part:R0010 R0010] -> OK


Digestion

  1. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  2. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  3. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  4. [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2006)


Sample No. 1~34
Sample DNA 100ng/μL×20200ng/μL×30
PstI 22
XbaI 2-
SpeI -2
Buffer 2 -5
Buffer 3 5-
BSA 55
dH2O 166
TOTAL 50μl50μl
--->Gel Check
Chiba-0829-4.JPG
Sample No. 1~34
Sample DNA 103
Loading Dye 22
dH2O -7
TOTAL 12μl12μl
insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026] : 685bp -> None
Chiba-0829-5.JPG
insert[http://partsregistry.org/Part:BBa_I9026 BBa_I9026]  : 745bp -> None
Chiba-0829-6.JPG
insert[http://partsregistry.org/BBa_S03154 BBa_S03154] : 685bp -> None
Chiba-0829-7.JPG
vector[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] : 2079bp -> OK


--->Gel Extract


--->Zymo Clean
->15μL
--->SAP
Sample No. 4
Sample DNA 15
SAP 2
SAP Buffer 3
dH2O 10
TOTAL 30μL


--->Zymo Clean
--->Suspension! (We got information, by the team-output, to the effect that the density of the sample is too low.)

Team:Output

TIME RESPONCE(solid)

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/29_August_2008"