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Team:Hawaii/Notebook/2008-06-26
From 2008.igem.org
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(updated notebook) |
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:*Ran gel but DNA did not migrate. | :*Ran gel but DNA did not migrate. | ||
:*Restriction digest of prep with EcoRI and ran gel. | :*Restriction digest of prep with EcoRI and ran gel. | ||
| + | |||
| + | ===Cell density count=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* Completed [[Team:Hawaii/PCC6803_cell_density|cell density count experiment]] | ||
| + | |||
| + | === Purified triparental conjugants=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* Restreaked transformed PCC6803 on new BG-11+sm<sub>2.5</sub>+sp<sub>2.5</sub> plates for purification | ||
== Drylab Work == | == Drylab Work == | ||
Revision as of 06:42, 27 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Large Scale Plasmid Prep
- Margaret, Krystle
- Found concentration using spectrophotometer
- Ran gel but DNA did not migrate.
- Restriction digest of prep with EcoRI and ran gel.
Cell density count
- Grace
- Completed cell density count experiment
Purified triparental conjugants
- Grace
- Restreaked transformed PCC6803 on new BG-11+sm2.5+sp2.5 plates for purification
Drylab Work
Design of Plasmid for Secretion Project
- Grace, Krystle
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Large Scale Plasmid Prep
- Margaret
- I am updating the wiki with the details of this experiment: the how to and the what went wrong.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
