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Team:Hawaii/Notebook/2008-06-26
From 2008.igem.org
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:<strong> Grace, Krystle </strong> | :<strong> Grace, Krystle </strong> | ||
| - | :* | + | :* Planned timeline (still in works) for secretion project |
| - | :* | + | :* Will convert pRL1383a into a BioBrick format plasmid so testing in PCC6803 can begin ASAP |
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| - | <blockquote>'' | + | <blockquote>''Oooh, look at the pretty colors'' - Krystle, refering to the agarose gel</blockquote> |
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
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Latest revision as of 06:46, 27 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Large Scale Plasmid Prep
- Margaret, Krystle
- Found concentration using spectrophotometer
- Ran gel but DNA did not migrate.
- Restriction digest of prep with EcoRI and ran gel.
Cell density count
- Grace
- Completed cell density count experiment
Purified triparental conjugants
- Grace
- Restreaked transformed PCC6803 on new BG-11+sm2.5+sp2.5 plates for purification
Drylab Work
Timeline for Secretion Project
- Grace, Krystle
- Planned timeline (still in works) for secretion project
- Will convert pRL1383a into a BioBrick format plasmid so testing in PCC6803 can begin ASAP
Large Scale Plasmid Prep
- Margaret
- I am updating the wiki with the details of this experiment: the how to and the what went wrong.
Discussion
Quote of the Day
Oooh, look at the pretty colors - Krystle, refering to the agarose gel
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
