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Team:Hawaii/Notebook/2008-10-25
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verification of plasmid preps=== :<strong> Grace</strong> [[Image:102508plasmiddigest.jpg|right|thumb|EtBr stained 4% a...) |
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:* RE digested all preps using EcoRI and PstI for 3 hours | :* RE digested all preps using EcoRI and PstI for 3 hours | ||
:* Ran on gel | :* Ran on gel | ||
| + | ===Inoculated 50ml TB+sp<sub>100</sub> with single colony BB1 for plasmid prep=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | ===Construction of BB-pRL1383a/K125820=== | ||
| + | <strong>Krystle</strong> | ||
| + | :* Restriction digest BB-pRL1383a with EcoRI and PstI | ||
| + | :* Dephosphorelated restriction product with SAP for 1 hour | ||
| + | :* Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase | ||
== Drylab Work == | == Drylab Work == | ||
| Line 17: | Line 25: | ||
:*Worked on presentation | :*Worked on presentation | ||
| + | ===parts=== | ||
| + | :<strong> Margaret</strong> | ||
| + | |||
| + | :*added parts to registry | ||
= Discussion = | = Discussion = | ||
Latest revision as of 00:21, 30 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of plasmid preps
- Grace
- PCR'd plasmid preps from yesterday
- RE digested all preps using EcoRI and PstI for 3 hours
- Ran on gel
Inoculated 50ml TB+sp100 with single colony BB1 for plasmid prep
- Grace
Construction of BB-pRL1383a/K125820
Krystle
- Restriction digest BB-pRL1383a with EcoRI and PstI
- Dephosphorelated restriction product with SAP for 1 hour
- Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase
Drylab Work
iGEM presentation
- Grace
- Worked on presentation
parts
- Margaret
- added parts to registry
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
