Team:Hawaii/Notebook/2008-10-29
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===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a|Antibiotic Test, round 2]]=== | ===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a|Antibiotic Test, round 2]]=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
- | [[Image:102908colonyPCR.png|right|thumb| | + | [[Image:102908colonyPCR.png|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Three microliters of PCR product were loaded into each lane.]] |
{|class=wikitable border=1 align=center | {|class=wikitable border=1 align=center | ||
|+ '''Colony Count of J33207+pRL1383a Transformants''' | |+ '''Colony Count of J33207+pRL1383a Transformants''' | ||
Line 13: | Line 13: | ||
!Blue? | !Blue? | ||
|- | |- | ||
- | |align=center|LB+Amp<sub>100</sub>X-gal (+) control | + | |align=center|LB+Amp<sub>100</sub>X-gal <br>(+) control |
|align=center|J33207 in pSB1A2 in DH5α | |align=center|J33207 in pSB1A2 in DH5α | ||
|align=center|too many to count, medium | |align=center|too many to count, medium | ||
|align=center|yes | |align=center|yes | ||
|- | |- | ||
- | |align=center|LB+sp<sub>100</sub>X-gal (+) control | + | |align=center|LB+sp<sub>100</sub>X-gal <br>(+) control |
- | |align=center|BBpRL1383a-1 (10/25 inoculation plasmid prep) in DH5α | + | |align=center|BBpRL1383a-1 <br>(10/25 inoculation plasmid prep) in DH5α |
|align=center|too many to count, small | |align=center|too many to count, small | ||
|align=center|no | |align=center|no | ||
|- | |- | ||
- | |align=center|LB+X-gal (+) control | + | |align=center|LB+X-gal <br>(+) control |
|align=center|untransformed DH5α | |align=center|untransformed DH5α | ||
|align=center|lawn/smear | |align=center|lawn/smear | ||
|align=center|no | |align=center|no | ||
|- | |- | ||
- | |align=center|LB+sp<sub>2.5</sub>+X-gal (-) control | + | |align=center|LB+sp<sub>2.5</sub>+X-gal <br>(-) control |
|align=center|untransformed DH5α | |align=center|untransformed DH5α | ||
|align=center|0 | |align=center|0 | ||
|align=center|no | |align=center|no | ||
|- | |- | ||
- | |align=center|LB+sp<sub>100</sub> (-) control | + | |align=center|LB+sp<sub>100</sub> <br>(-) control |
|align=center|untransformed DH5α | |align=center|untransformed DH5α | ||
|align=center|0 | |align=center|0 |
Latest revision as of 01:20, 30 October 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic Test, round 2
- Grace
Plate | Cells | Colonies | Blue? |
---|---|---|---|
LB+Amp100X-gal (+) control | J33207 in pSB1A2 in DH5α | too many to count, medium | yes |
LB+sp100X-gal (+) control | BBpRL1383a-1 (10/25 inoculation plasmid prep) in DH5α | too many to count, small | no |
LB+X-gal (+) control | untransformed DH5α | lawn/smear | no |
LB+sp2.5+X-gal (-) control | untransformed DH5α | 0 | no |
LB+sp100 (-) control | untransformed DH5α | 0 | no |
LB+sp2.5 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
LB+sp5 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
LB+sp10 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
LB+sp20 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
LB+sp25 | J33207+pRL1383a ligation in DH5α | lawn/smear | no |
LB+sp40 | J33207+pRL1383a ligation in DH5α | too many to count, small | no |
LB+sp50 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
LB+sp75 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
LB+sp100 | J33207+pRL1383a ligation in DH5α | too many to count, small to medium | no |
- Colony PCR of J33207, BB-1, and sp75, 100transformants
Drylab Work
Project page
- Grace
- Updated materials, methods and results for Project Part B.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]