Team:NTU-Singapore/Notebook/30 June 2008

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Chin Chong
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=Monday 30 June:=
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==Chin Chong==
*Stock check of items purchased so far and shifted them into new lab with Darius's help
*Stock check of items purchased so far and shifted them into new lab with Darius's help
*Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks  
*Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks  
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*Did inoculation of colonies from Top cells containing:  
*Did inoculation of colonies from Top cells containing:  
#LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
#LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
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#Standard promoter [http://partsregistry.org/Part:BBa_J23101 BBa_J23101]  
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#Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]  
#GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],  
#GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],  
#Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and  
#Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and  
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**This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
**This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
**M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB
**M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB
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==Choon Kit, Zhen Fu,Hung:==
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*Nanodrop to measure concentrations and purities of pFe,pLacI,GFP,RBS32,RBS34,pT7,Terminator. The data show a ratio of A260/A280 varying around 1.85 to 1.95, which mean the purities are quite good.
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Latest revision as of 02:23, 28 October 2008

Monday 30 June:

Chin Chong

  • Stock check of items purchased so far and shifted them into new lab with Darius's help
  • Received quotation for LsrA-RBS and Lysis sequencing from Science Werke, confirmed the purchase and sequence should arrive in 2-4 weeks
  • Purchased items from SBS general store
  • Did inoculation of colonies from Top cells containing:
  1. LacI-GFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004],
  2. Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]
  3. GFP reporter devices with Weak [http://partsregistry.org/Part:BBa_J23150 BBa_J23150],
  4. Medium [http://partsregistry.org/Part:BBa_J23151 BBa_J23151] and
  5. Strong [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] promoters
  • Incubate cells in 5ml of LB broth containing the respective anitbiotics and allow them to grow overnight
    • This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead of LB broth
    • M9 medium with glycerol will be introduced in the following day after the cells have been allowed to grow overnight in LB

Choon Kit, Zhen Fu,Hung:

  • Nanodrop to measure concentrations and purities of pFe,pLacI,GFP,RBS32,RBS34,pT7,Terminator. The data show a ratio of A260/A280 varying around 1.85 to 1.95, which mean the purities are quite good.
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