"
Team:Waterloo
From 2008.igem.org
(Difference between revisions)
(→Our Project: A plasmid-safe, inducible genome-degradation strain for post-kill gene expression) |
|||
| (16 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
| - | {| style="color:#1b2c8a;background-color:# | + | |
| + | |||
| + | |||
| + | {| style="border-spacing:0px; border-width:thick; border-color:black; border-style:solid; width:100%" | ||
| + | |- | ||
| + | | style="font-size:600%; padding:50px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" | '''University of Waterloo''' | ||
| + | |- | ||
| + | |style="font-size:200%; padding:15px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" | '''Genome-free Bacterial Bioproduct Factory:''' | ||
| + | A plasmid-safe, inducible genome-degradation strain for post-kill gene expression | ||
| + | |- | ||
| + | |style="font-size:130%; padding:5px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" | | ||
| + | '''''Code Name: The Headless Chicken Project''''' | ||
| + | |}<br> | ||
| + | {| style="color:#1b2c8a;background-color:#FBCC30;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
!align="center"|[[Team:Waterloo|Home]] | !align="center"|[[Team:Waterloo|Home]] | ||
!align="center"|[[Team:Waterloo/Team|The Team]] | !align="center"|[[Team:Waterloo/Team|The Team]] | ||
| Line 10: | Line 23: | ||
!align="center"|[[Team:Waterloo/Sponsors|Sponsors]] | !align="center"|[[Team:Waterloo/Sponsors|Sponsors]] | ||
|} | |} | ||
| - | |||
<table border="0"> | <table border="0"> | ||
<tr> | <tr> | ||
| Line 19: | Line 31: | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | <br> | ||
| + | {| style="border-spacing:0px; border-width:thick; border-color:black; border-style:solid" | ||
| + | |- | ||
| + | | style="font-size:large; border-bottom-width:thick; border-bottom-color:black; border-bottom-style:solid; padding:5px; background-color:#FBCC30" colspan="2" | '''Genome-free Bacterial Bioproduct Factory:''' | ||
| + | A plasmid-safe, inducible genome-degradation strain for post-kill gene expression | ||
| + | |||
| + | |- | ||
| + | | style="padding:20px" | | ||
| + | '''''Code Name: The Headless Chicken Project''''' | ||
| + | |||
| + | |||
| + | '''Abstract''' | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
The aim of our project is to engineer a genome-free, cell-based expression system capable of producing a desired protein or activating a pathway in response to an environmental signal. Genome degradation is achieved using the combined activity of a restriction endonuclease to fragment the genome and an exonuclease to hasten degradation. The gene for the protein of interest will be located in a plasmid lacking recognition sites for the endonuclease, allowing it to remain intact after genome degradation. The plasmid genes will be expressed using the remaining cell resources until they expire. The primary application of this design would be an ''in situ'' compound production and delivery system for agricultural, industrial or therapeutic use to continue for a period of time. | The aim of our project is to engineer a genome-free, cell-based expression system capable of producing a desired protein or activating a pathway in response to an environmental signal. Genome degradation is achieved using the combined activity of a restriction endonuclease to fragment the genome and an exonuclease to hasten degradation. The gene for the protein of interest will be located in a plasmid lacking recognition sites for the endonuclease, allowing it to remain intact after genome degradation. The plasmid genes will be expressed using the remaining cell resources until they expire. The primary application of this design would be an ''in situ'' compound production and delivery system for agricultural, industrial or therapeutic use to continue for a period of time. | ||
| - | + | | style="padding:5px" | [[Image:WikiArt600.png|400px|right]] | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | |} | |
| - | {| style="width:100%; | + | |
| + | |||
| + | {| style="border-spacing:0px; border-width:thick; border-color:black; border-style:solid; width:100%" | ||
| + | |- | ||
| + | | style="font-size:large; border-bottom-width:thick; border-bottom-color:black; border-bottom-style:solid; padding:5px; background-color:#FBCC30" colspan="2" | [[Team:Waterloo/Sponsors|Gold Level Support]] Provided By | ||
|- | |- | ||
| - | |style=" | + | | style="padding:20px; text-align:center"| <html><a href="http://www.mef.uwaterloo.ca/"><img src="https://static.igem.org/mediawiki/2008/3/32/MEF.PNG" width="300px" height="90px"><br> |
Mathematics Endowment Fund</a></html> | Mathematics Endowment Fund</a></html> | ||
| - | |style=" | + | | style="padding:20px"; text-align:center|<html><a href="http://www.science.uwaterloo.ca/"><img src="https://static.igem.org/mediawiki/2008/8/85/SciFac.PNG" width="300px" height="90px"><br> |
Faculty of Science</a></html> | Faculty of Science</a></html> | ||
|} | |} | ||
Latest revision as of 04:00, 30 October 2008
| University of Waterloo | |
| Genome-free Bacterial Bioproduct Factory:
A plasmid-safe, inducible genome-degradation strain for post-kill gene expression | |
|
Code Name: The Headless Chicken Project |
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Sponsors |
|---|
 |
The University of Waterloo 2008 iGEM Team is an interdisciplinary, undergraduate-run group spanning the Faculties of Science, Mathematics, and Engineering. Our undergraduate members and graduate and faculty advisors bring skills, expertise, and perspectives from a broad range of fields, including Biology, Biochemistry, Computer Science, Bioinformatics, Electrical and Computer Engineering, Chemical Engineering, and Mathematical Physics. |
| Genome-free Bacterial Bioproduct Factory:
A plasmid-safe, inducible genome-degradation strain for post-kill gene expression | |
|
Code Name: The Headless Chicken Project
The aim of our project is to engineer a genome-free, cell-based expression system capable of producing a desired protein or activating a pathway in response to an environmental signal. Genome degradation is achieved using the combined activity of a restriction endonuclease to fragment the genome and an exonuclease to hasten degradation. The gene for the protein of interest will be located in a plasmid lacking recognition sites for the endonuclease, allowing it to remain intact after genome degradation. The plasmid genes will be expressed using the remaining cell resources until they expire. The primary application of this design would be an in situ compound production and delivery system for agricultural, industrial or therapeutic use to continue for a period of time. |  |
| Gold Level Support Provided By | |
 Mathematics Endowment Fund |  Faculty of Science |
