Team:ESBS-Strasbourg/Parts

From 2008.igem.org

(Difference between revisions)
(Prototype team page)
(→Toolbox for modular transcription factors)
 
(17 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
 +
<div id="maincontent" style="margin-top:150px;">
 +
__notoc__
-
<html>
+
=Parts submitted=
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
Following you can find a summary of our contribution to the registry.
 +
==Toolbox for modular transcription factors==
 +
[[image:ESBS_TF.jpg|thumb|300px|right]]
 +
===DNA binding domains===
 +
* '''tetR''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
 +
* '''cI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
 +
* '''lexA''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
 +
* '''lacI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
 +
* '''Gal4''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]
 +
===Activation domain===
 +
* '''VP16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]
 +
===Repression domain===
 +
* '''TUP1''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]
 +
===Fluorescent markers===
 +
* '''CFP''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
 +
* '''mCherry''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
 +
* '''mOrange''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]
 +
===Junctions===
 +
* '''linker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]
 +
===Tags===
 +
* '''cin8 - cell cycle dependent degradation ''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
 +
* '''hsl1 – cell cycle dependent degradation''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]
 +
<br>
 +
=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]
 +
<br>
 +
<br>
 +
==Toolbox for modular promoters==
 +
===Minimal promoters of different strength===
 +
* '''cyc100''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
 +
* '''cyc70''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
 +
* '''cyc43''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
 +
* '''cyc28''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
 +
* '''cyc16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]
 +
===Operator sites===
 +
* '''tetR operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
 +
* '''cI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
 +
* '''lexA operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
 +
* '''lacI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
 +
* '''Gal4 operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]
 +
<br>
-
{|align="justify"
+
==Yeast selection markers==
-
|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
+
* '''Leu2 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
-
|[[Image:Example_logo.png|200px|right|frame]]
+
* '''Ura3 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]
-
|-
+
<br>
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:Team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:ESBS-Strasbourg | Team Example 2]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
+
==Remakes of good, but not available BioBricks==
-
 
+
* '''Gal1 promoter''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
* '''Adh1 terminator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]
-
!align="center"|[[Team:ESBS-Strasbourg|Home]]
+
<br>
-
!align="center"|[[Team:ESBS-Strasbourg/Team|The Team]]
+
-
!align="center"|[[Team:ESBS-Strasbourg/Project|The Project]]
+
-
!align="center"|[[Team:ESBS-Strasbourg/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:ESBS-Strasbourg/Modeling|Modeling]]
+
-
!align="center"|[[Team:ESBS-Strasbourg/Notebook|Notebook]]
+
-
|}
+
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+
-
 
+
-
 
+
-
===Note===
+
-
 
+
-
If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
+

Latest revision as of 23:07, 29 October 2008


Parts submitted

Following you can find a summary of our contribution to the registry.

Toolbox for modular transcription factors

ESBS TF.jpg

DNA binding domains

  • tetR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
  • cI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
  • lexA [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
  • lacI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
  • Gal4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]

Activation domain

  • VP16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]

Repression domain

  • TUP1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]

Fluorescent markers

  • CFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
  • mCherry [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
  • mOrange [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]

Junctions

  • linker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]

Tags

  • cin8 - cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
  • hsl1 – cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]


=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]

Toolbox for modular promoters

Minimal promoters of different strength

  • cyc100 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
  • cyc70 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
  • cyc43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
  • cyc28 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
  • cyc16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]

Operator sites

  • tetR operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
  • cI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
  • lexA operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
  • lacI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
  • Gal4 operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]


Yeast selection markers

  • Leu2 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
  • Ura3 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]


Remakes of good, but not available BioBricks

  • Gal1 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
  • Adh1 terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]

Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/Parts"