Team:ESBS-Strasbourg/Parts

From 2008.igem.org

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__notoc__
=Parts submitted=
=Parts submitted=
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None yet... :(
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Following you can find a summary of our contribution to the registry.
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==Toolbox for modular transcription factors==
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[[image:ESBS_TF.jpg|thumb|300px|right]]
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===DNA binding domains===
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* '''tetR''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
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* '''cI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
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* '''lexA''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
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* '''lacI''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
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* '''Gal4''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]
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===Activation domain===
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* '''VP16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]
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===Repression domain===
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* '''TUP1''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]
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===Fluorescent markers===
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* '''CFP''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
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* '''mCherry''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
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* '''mOrange''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]
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===Junctions===
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* '''linker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]
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===Tags===
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* '''cin8 - cell cycle dependent degradation ''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
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* '''hsl1 – cell cycle dependent degradation''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]
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<br>
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=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]
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<br>
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<br>
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===Note===
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==Toolbox for modular promoters==
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===Minimal promoters of different strength===
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* '''cyc100''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
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* '''cyc70''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
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* '''cyc43''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
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* '''cyc28''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
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* '''cyc16''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]
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===Operator sites===
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* '''tetR operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
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* '''cI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
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* '''lexA operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
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* '''lacI operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
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* '''Gal4 operator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]
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<br>
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here. This is not necessary but it may be a nice list to keep track of.
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==Yeast selection markers==
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* '''Leu2 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
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* '''Ura3 - autotrophy marker''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]
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<br>
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==Remakes of good, but not available BioBricks==
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* '''Gal1 promoter''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
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* '''Adh1 terminator''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]
<br>
<br>
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<div id="bottom">{{Template:Team:ESBS-Strasbourg/Templates/Navigation}}</div>
 

Latest revision as of 23:07, 29 October 2008


Parts submitted

Following you can find a summary of our contribution to the registry.

Toolbox for modular transcription factors

ESBS TF.jpg

DNA binding domains

  • tetR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105003 BBa_K105003]
  • cI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105004 BBa_K105004]
  • lexA [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105005 BBa_K105005]
  • lacI [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105006 BBa_K105006]
  • Gal4 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105007 BBa_K105007]

Activation domain

  • VP16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105001 BBa_K105001]

Repression domain

  • TUP1 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105002 BBa_K105002]

Fluorescent markers

  • CFP [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105009 BBa_K105009]
  • mCherry [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105010 BBa_K105010]
  • mOrange [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105011 BBa_K105011]

Junctions

  • linker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105012 BBa_K105012]

Tags

  • cin8 - cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105013 BBa_K105013]
  • hsl1 – cell cycle dependent degradation [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105015 BBa_K105015]


=> all these parts are in our new plasmid derivate [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105000 pSB1A2_fusion]

Toolbox for modular promoters

Minimal promoters of different strength

  • cyc100 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105027 BBa_K105027]
  • cyc70 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105028 BBa_K105028]
  • cyc43 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105029 BBa_K105029]
  • cyc28 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105030 BBa_K105030]
  • cyc16 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105031 BBa_K105031]

Operator sites

  • tetR operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105020 BBa_K105020]
  • cI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105021 BBa_K105021]
  • lexA operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105022 BBa_K105022]
  • lacI operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105023 BBa_K105023]
  • Gal4 operator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105024 BBa_K105024]


Yeast selection markers

  • Leu2 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105016 BBa_K105016]
  • Ura3 - autotrophy marker [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105017 BBa_K105017]


Remakes of good, but not available BioBricks

  • Gal1 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105026 BBa_K105026]
  • Adh1 terminator [http://partsregistry.org/wiki/index.php?title=Part:BBa_K105025 BBa_K105025]

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