Team:Valencia

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[[Team:Valencia/Project|<font color="#000000">Hot Yeast</font>]]
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Valencia iGEM '08 Team heats it up in your lab!!!
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Tired of forgetting to keep our plates on the stove and therefore losing all of our work we thought, wouldn’t it be great to have culture that heats itself up? This idea was the basis for our project.
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We aim to express transmembrane protein UCP in Saccharomyces cerevisiae and be able to record a temperature increase using a calorimeter. In a second phase, we will implement a regulatory system for the temperature from both an upper and lower limit.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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Abstract
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The present project aims to demonstrate that the temperature of a microbial culture might be modulated through the expression of the mammalian uncoupling protein UCP-1. Saccharomyces cerevisiae strains genetically modified to express wild type UCP-1, mutant sequences with increased uncoupling activity, as well as a control strain were cultured in an Liquid Culture Calorimeter (LCC) we developed. The system consisted of a modified thermo flask with an inserted thermocouple allowing real-time accurate temperature measurements. Different conditions, such as initial densities, amounts of inductor, or shaking speeds were tested. We succeeded to obtain significant temperature increases in the mutant strains compared with the other strains. We also developed an effective model of our system. Although the system is not always stable and might be sensitive to external perturbations, this is the first time a significant increase in temperature associated to UCP-1 expression in yeast is reported.  
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your Team:Example namespace. 
 
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Acknowledgements
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* [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial] ([http://www.cib.csic.es/es/grupo.php?idgrupo=39 CIB], Mitochondrial Bioenergetics, [http://www.csic.es/index.do?lengua=en CSIC])
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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* [http://www.uv.es Universitat de València]
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* [http://www.upv.es Universitat Politècnica de València]
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* [http://www.etsii.upv.es Escuela Tecnica Superior de Ingenieros Industriales de la Universidad Politecnica de Valencia]
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* Vicerectorado de Investigacion desarrollo e innovacion de la Universidad Politecnica de Valencia.
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''Tell us more about your project. Give us background. Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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* [http://www.uv.es/cavanilles Institut Cavanilles de la Universitat de Valencia]
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* Project Ingenio mathematica [http://www.i-math.org/?q=en i-math]
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* European community's Seventh Framework Programme (FP7/2007-2013) under /grant agreement/ nº 212894. Targeting enviromental pollution with engineered microbial systems ''à la carte'' ([http://gydb.uv.es/tarpol/ TARPOL])
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|align="center"|[[Team:Valencia | Team Example 2]]
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!align="center"|[[Team:Valencia|Home]]
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!align="center"|[[Team:Valencia/Team|The Team]]
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!align="center"|[[Team:Valencia/Project|The Project]]
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!align="center"|[[Team:Valencia/Modeling|Modeling]]
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!align="center"|[[Team:Valencia/Notebook|Notebook]]
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[[Team:Valencia/Team|<font color="#000000">'''The Team'''</font>]]
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[[Team:Valencia/Notebook|<font color="#000000">'''Lab Notebook'''</font>]]
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| colspan="7" style="background:#FFB428;" | <Center>[[Team:Valencia/Notebook/October|<font color="#047DB5">October</font>]]</Center>
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| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
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| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp;[[Team:Valencia/Notebook/October#October 1st | 1]]|| &nbsp;[[Team:Valencia/Notebook/October#October 2nd | 2]] || &nbsp;3 || &nbsp;4 || &nbsp;5
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| &nbsp;6 || &nbsp;[[Team:Valencia/Notebook/October#October 7th |7]]  || &nbsp;&nbsp;8|| 9 || 10 || 11|| 12
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| [[Team:Valencia/Notebook/October#October 13th |13]] || [[Team:Valencia/Notebook/October#October 14th |14]]  || &nbsp;15 || 16 || [[Team:Valencia/Notebook/October#October 17th |17]] || 18 || 19
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| [[Team:Valencia/Notebook/October#October 20th |20]] || 21 || &nbsp;22 || [[Team:Valencia/Notebook/October#October 23rd |23]]  || [[Team:Valencia/Notebook/October#October 24th |24]]  || 25 || 26
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| [[Team:Valencia/Notebook/October#October 27th |27]]  || 28 || &nbsp;29 || 30 || 31 || <Font Color="#c0c0c0">&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
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[[Image:Hot_yeast_prueba_naranja.png|40px]]&nbsp;&nbsp;&nbsp;&nbsp;[[Team:Valencia/Notebook/Pictures |<font color="#047DB5">'''Lab Pictures!'''</font>]]</div></div></div>
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[http://www.intertech.upv.es/publications_media.htm <font color="#000000">'''Valencia Team in Press'''</font>]
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Latest revision as of 10:07, 30 October 2008


Valencia iGEM '08 Team heats it up in your lab!!!

Tired of forgetting to keep our plates on the stove and therefore losing all of our work we thought, wouldn’t it be great to have culture that heats itself up? This idea was the basis for our project.

We aim to express transmembrane protein UCP in Saccharomyces cerevisiae and be able to record a temperature increase using a calorimeter. In a second phase, we will implement a regulatory system for the temperature from both an upper and lower limit.



Abstract

The present project aims to demonstrate that the temperature of a microbial culture might be modulated through the expression of the mammalian uncoupling protein UCP-1. Saccharomyces cerevisiae strains genetically modified to express wild type UCP-1, mutant sequences with increased uncoupling activity, as well as a control strain were cultured in an Liquid Culture Calorimeter (LCC) we developed. The system consisted of a modified thermo flask with an inserted thermocouple allowing real-time accurate temperature measurements. Different conditions, such as initial densities, amounts of inductor, or shaking speeds were tested. We succeeded to obtain significant temperature increases in the mutant strains compared with the other strains. We also developed an effective model of our system. Although the system is not always stable and might be sensitive to external perturbations, this is the first time a significant increase in temperature associated to UCP-1 expression in yeast is reported.



Acknowledgements

  • [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6 Eduardo Rial] ([http://www.cib.csic.es/es/grupo.php?idgrupo=39 CIB], Mitochondrial Bioenergetics, [http://www.csic.es/index.do?lengua=en CSIC])
  • [http://www.uv.es Universitat de València]
  • [http://www.upv.es Universitat Politècnica de València]
  • [http://www.etsii.upv.es Escuela Tecnica Superior de Ingenieros Industriales de la Universidad Politecnica de Valencia]
  • Vicerectorado de Investigacion desarrollo e innovacion de la Universidad Politecnica de Valencia.
  • [http://www.uv.es/cavanilles Institut Cavanilles de la Universitat de Valencia]
  • Project Ingenio mathematica [http://www.i-math.org/?q=en i-math]
  • European community's Seventh Framework Programme (FP7/2007-2013) under /grant agreement/ nº 212894. Targeting enviromental pollution with engineered microbial systems à la carte ([http://gydb.uv.es/tarpol/ TARPOL])



Valencia sponsors.png
Team picture.jpg


October
Mo Tu We Th Fr Sa Su
29 30    1   2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2

Hot yeast prueba naranja.png    Lab Pictures!


[http://www.intertech.upv.es/publications_media.htm Valencia Team in Press]

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