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Alberta NINT/14 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/11_July_2008 | < Previous entry]] | + | [[Alberta_NINT/11_July_2008 | < Previous entry]] | [[Alberta_NINT/15_July_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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XL1-B (30 C) 0.147 0.238 0.344 0.345 | XL1-B (30 C) 0.147 0.238 0.344 0.345 | ||
| - | + | lab work (JD): | |
| + | Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations | ||
| + | Plate - # colonies | ||
| + | K102031W - - 2 | ||
| + | K102021W - - 1 | ||
| + | NegativeW- - 2 | ||
| + | K102031J - - 0 | ||
| + | K102021J - - 0 | ||
| + | NegativeJ - -0 | ||
| + | Obviously, weekends ligations didn't work either. | ||
Latest revision as of 22:59, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Growth rates of cells containing the plasmids K102022, K102032, K102032+K102011, K102032+K102034,
K102032+K102035, K102032+K102036 and XL1-B were to be determined by measuring the cell count every
hour for cells grown at 30 and 37 C. Growth was only observed in overnights for K102022 and XL1-B.
t=1 hr t=2 hr t=3 hr t=4 hr
LB 0.000 0.000 0.000 0.000
K102022 (30 C) 0.128 0.162 0.192 0.247
K102022 (37 C) 0.167 0.278 0.351 0.407
XL1-B (30 C) 0.153 0.166 0.194 0.230
XL1-B (30 C) 0.147 0.238 0.344 0.345
lab work (JD):
Nothing grew from Friday's ligations so Wayne re-tranformed my ligations and carried out his own ligations
Plate - # colonies
K102031W - - 2
K102021W - - 1
NegativeW- - 2
K102031J - - 0
K102021J - - 0
NegativeJ - -0
Obviously, weekends ligations didn't work either.
