Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

(Difference between revisions)
(→Solutions and buffers)
(→Protocols)
 
(18 intermediate revisions not shown)
Line 1: Line 1:
<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
<div id="header">{{Template:Team:ESBS-Strasbourg/Templates/Header}}</div>
-
<div id="maincontent" style="margin-top:170px;">
+
<div id="maincontent" style="margin-top:150px;">
-
[[Team:ESBS-Strasbourg/Project|<= back]]
+
-
<br>
+
-
==E.Coli==
+
__NOTOC__
-
 
+
-
*'''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br>
+
-
 
+
-
*'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol] <br>
+
-
 
+
-
*'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br>
+
 +
=Protocols=
<br>
<br>
{|
{|
|-valign="top"
|-valign="top"
-
|width="475px"|
+
|width="360px"|
 +
==E.Coli==
 +
 
 +
*[[Team:ESBS-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br>
 +
*[http://openwetware.org/wiki/TOP10_chemically_competent_cells Preparation of TOP10 chemically competent cells (iGEM)] <br>
 +
*[[Team:ESBS-Strasbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br>
 +
*[[Team:ESBS-Strasbourg/Transformation Heat shock|Heat shock transformation]]<br>
 +
 
 +
|width="360px"|
==Cloning==
==Cloning==
*[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
*[[Team:ESBS-Strasbourg/PCR|PCR]] <br>
Line 26: Line 27:
*[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br>
*[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br>
*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br>
-
 
+
*[[Team:ESBS-Strasbourg/Concentrations Measurements|Concentrations Measurements]] <br>
-
|width="475px"|
+
|width="360px"|
==Solutions and buffers==
==Solutions and buffers==
-
*'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
+
*[http://openwetware.org/wiki/SOB SOB for the preparation of competent cells] <br>
-
*'''SOC''' for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC] <br>
+
*[http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes] <br>
-
*'''TENS''' for the do it yourself MiniPrep [[Team:ESBS-Strasbourg/TENS|TENS]] <br>
+
*[[Team:ESBS-Strasbourg/TENS|TENS for the do it yourself MiniPrep]] <br>
|}
|}
 +
<br>
 +
 +
 +
----
 +
 +
Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]
<br>
<br>
-
<div id="bottom">{{Template:Team:ESBS-Strasbourg/Templates/Navigation}}</div>
 

Latest revision as of 00:13, 29 October 2008


Protocols


E.Coli

Cloning

Solutions and buffers

  • [http://openwetware.org/wiki/SOB SOB for the preparation of competent cells]
  • [http://openwetware.org/wiki/SOC SOC for bacterial transformation purposes]
  • TENS for the do it yourself MiniPrep



Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]


Retrieved from "http://2008.igem.org/Team:ESBS-Strasbourg/Protocols"